A multispectral fundus camera (MSFC), as a novel noninvasive technology, uses an extensive range of monochromatic light sources that enable the view of different sectional planes of the retinal and choroidal structures. However, MSFC imaging involves complex processes affected by various factors, and the recognized theory based on light absorption above the choroid is not sufficient. In an attempt to supplement the relevant explanations, in this study, we used optical coherence tomography (OCT), a three-dimensional tomography modality, to analyze MSFC results at the retina and choroid. The swept-source OCT system at 1060 nm wavelength with a 200 kHz A-scan rate and an MSFC with 11 bands at 470 to 845 nm are employed. A quantitative evaluation procedure is proposed to compare MSFC and OCT en face images. The comparative study shows that 1) the MSFC images with the illumination wavelength of less than 605 nm could mainly provide the retinal structure information; 2) Relative choroidal layer thickness information could be inferred from the MSFC images, especially the image acquiring under the wavelength more than 605 nm. According to the results, further investigation revealed the contribution of the perivascular tissue and the sclera scattering in the difference of vascular brightness in MSFC images.

中文翻译：

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基于光学相干断层扫描的多波长眼底成像的脉络膜脉络膜血管成像

作为一种新型的非侵入性技术，多光谱眼底照相机（MSFC）使用了范围广泛的单色光源，可以观察到视网膜和脉络膜结构的不同截面。但是，MSFC成像涉及受各种因素影响的复杂过程，并且基于脉络膜上方光吸收的公认理论还不够。为了补充相关解释，在本研究中，我们使用了三维相干断层扫描光学相干断层扫描（OCT）来分析视网膜和脉络膜上的MSFC结果。使用了具有200 kHz A扫描速率的1060 nm波长的扫频OCT系统和470至845 nm处的11个波段的MSFC。定量评价方法，提出了比较MSFC和OCT恩面对图片。对比研究表明：1）照射波长小于605 nm的MSFC图像可主要提供视网膜结构信息；2）可以从MSFC图像中推断出脉络膜相对厚度信息，尤其是在大于605 nm的波长下获取的图像。根据结果​​，进一步的研究揭示了MSFC图像中血管周围组织和巩膜散射对血管亮度差异的贡献。