The technique of high-speed countercurrent chromatography (HSCCC) was applied to the preparative isolation and purification of liquiritin and glycyrrhizic acid from a crude extract of Glycyrrhiza uralensis Fisch for the first time. Using single factor and orthogonal design experiments, the best extraction conditions were 70% ethanol, 1:25 ratio of solid-to-liquid (w/v) and extracted 1.5 h at 80°C. The contents of liquiritin and glycyrrhizic acid in the crude extract were 1.3 and 5.3%, respectively. Using the two-phase solvent system of ethyl acetate–methanol–water (5:2:5, v/v), 6.0 mg liquiritin (the purity was 96.7%, and the recovery was 89.3%), and 20.5 mg glycyrrhizic acid (the purity was 98.9%, and the recovery was 77.1%) were obtained from 500 mg crude extraction by HSCCC, respectively. The retention rate of stationary phase was 51.0%. Their structures were identified by high-performance liquid chromatography, melting points, ultraviolet radiation, Fourier transform infrared (FTIR), Electrospray ionization mass spectrometry (ESI-MS), ¹H Nuclear Magnetic Resonance (NMR) and ¹³C NMR spectra. The scavenging abilities of glycyrrhizic acid to 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals were stronger than those of liquiritin.

中文翻译：

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高速逆流色谱法从甘草中分离和纯化甘草酸和甘草酸。

高速逆流色谱（HSCCC）技术用于甘草粗提液中甘草酸和甘草酸的制备分离纯化第一次是Fisch。使用单因素和正交设计实验，最佳提取条件为70％乙醇，固液比1:25（w / v），并在80°C提取1.5 h。粗提物中的液体白蛋白和甘草次酸的含量分别为1.3％和5.3％。使用乙酸乙酯-甲醇-水（5：2：5，v / v）的两相溶剂系统，6.0 mg液体白蛋白（纯度为96.7％，回收率为89.3％）和20.5 mg甘草酸（从500毫克HSCCC粗提物中分别获得98.9％的纯度和77.1％的回收率。固定相的保留率为51.0％。通过高效液相色谱，熔点，紫外线，傅立叶变换红外（FTIR），电喷雾电离质谱（ESI-MS），¹ H核磁共振（NMR）和¹³ C NMR光谱。甘草酸对1，1-二苯基-2-吡咯并肼基和羟基自由基的清除能力强于甘草酸。