Halyomorpha halys (Stål), the brown marmorated stink bug, is an invasive and highly polyphagous insect that has caused serious economic injury to specialty and row crops in the United States and Europe. Here, we evaluated the effects of marking adult and nymphal H. halys with four different colors of fluorescent powder (Blaze Orange, Corona Pink, Horizon Blue, and Signal Green) on mobility and survivorship in laboratory bioassays. Adults and nymphs were marked using liquified fluorescent powder solutions and allowed to dry prior to bioassay. The presence of the marking solution had no significant effects on adult or nymphal mobility, adult survivorship, nymphal development, or adult flight capacity. We also evaluated the persistence of neon marker applied to the pronotum of H. halys adults and found this technique remained detectable for 2 wk under field conditions. Although both marking techniques are inexpensive, persist for ≥1 wk, and do not affect mortality, the neon marker method is more time-consuming, taking ~12 times longer to mark 50 adult H. halys compared with the liquified fluorescent powders. Thus, we would recommend using fluorescent powders for large-scale mark-release-recapture studies.

中文翻译：

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标记方法对Halyomorpha halys（半翅目：蝽科）成虫和若虫活动性、生存和田间恢复的影响


Halyomorpha halys (Stål) 是一种棕色有纹蝽，是一种入侵性的高度杂食性昆虫，对美国和欧洲的特种作物和中耕作物造成了严重的经济损失。在这里，我们评估了在实验室生物测定中用四种不同颜色的荧光粉（火焰橙、电晕粉、地平线蓝和信号绿）标记成虫和若虫 H. halys 对移动性和存活率的影响。使用液化荧光粉溶液标记成虫和若虫，并在生物测定前干燥。标记溶液的存在对成虫或若虫的活动能力、成虫存活率、若虫发育或成虫飞行能力没有显着影响。我们还评估了应用于 H. halys 成虫前胸背板上的霓虹灯标记的持久性，发现该技术在野外条件下仍可检测 2 周。虽然这两种标记技术都很便宜，持续时间≥1周，并且不影响死亡率，但霓虹灯标记方法更耗时，与液体荧光粉相比，标记 50 只成年 H. halys 所需的时间长约 12 倍。因此，我们建议使用荧光粉进行大规模标记-释放-回收研究。