ABSTRACT

Fifteen percent third-degree burn rat model was used to identify miRNAs that are markers of burn injury-induced myocardial damage. Cardiac tissues were evaluated to determine miRNA profile sequencing. Pearson’s correlation analysis was used between miRNAs and injury markers. ROC curve analysis was used to estimate miRNA’s sensitivity and specificity for the diagnosis of myocardial damage caused by burn injury. The sequencing analysis revealed 23 differentially expressed miRNAs. Pearson’s correlation analysis revealed that rno-miR-190b-3p and C5b9, rno-miR-341, rno-miR-344b-3p and TnI, rno-miR-344b-3p and CK-MB were significantly positively correlated, respectively. ROC curve analysis demonstrated that rno-miR-341, rno-miR-344b-3p, and rno-miR-190b-3p exhibited high sensitivity and specificity for the diagnosis of myocardial damage caused by burn injury. In conclusion, our results suggest that rno-miR-341, rno-miR-344b-3p, and rno-miR-190b-3p have the potential to be used as sensitive and specific biomarkers to diagnose myocardial damage caused by burn injury.

摘要

使用15％的三度烧伤大鼠模型来鉴定miRNA，这些miRNA是烧伤引起的心肌损伤的标志。评价心脏组织以确定miRNA谱图测序。在miRNA和损伤标记之间进行了Pearson相关分析。使用ROC曲线分析来评估miRNA在诊断烧伤引起的心肌损伤中的敏感性和特异性。测序分析揭示了23个差异表达的miRNA。皮尔森的相关分析表明，rno-miR-190b-3p和C5b9，rno-miR-341，rno-miR-344b-3p和TnI，rno-miR-344b-3p和CK-MB分别呈显着正相关。ROC曲线分析表明rno-miR-341，rno-miR-344b-3p，rno-miR-190b-3p和rno-miR-190b-3p对烧伤引起的心肌损伤的诊断具有很高的敏感性和特异性。总之，我们的结果表明，rno-miR-341，rno-miR-344b-3p和rno-miR-190b-3p有潜力用作诊断烧伤引起的心肌损伤的敏感和特异性生物标志物。