Prolonged, uninterrupted sitting is demonstrated to acutely impair glucose homeostasis, but also leads detrimental cardiovascular health effects. We examined whether ischemic preconditioning (IPC) prevents the impact of prolonged sitting-induced glucose intolerance, and measured related influencing factors such as (para)sympathetic nerve activity (assessed by heart rate variability (HRV)) and blood pressure during 2h prolonged sitting. In this randomized, controlled cross-over study, 15 healthy participants (80% men) with a mean age of 21±1 years (means±SD) and body mass index of 25.0±2.4 kg m² performed IPC (IPC; 4×5-min 220-mmHg unilateral occlusion at the thigh muscle) or a sham intervention (Sham; 4×5-min 20-mmHg), followed by 2h sitting. After IPC or Sham intervention, fingertip blood glucose was measured before and after 30, 60, 90, and 120 min of 75 g of glucose ingestions. Blood glucose responses during an oral glucose tolerance test was significantly attenuated, resulting in a lower area under the curve when sitting was preceded by a bout of IPC than Sham (P<0.05). IPC increased high-frequency oscillations and decreased the ratio of low-frequency to high-frequency oscillations at 120 min in HRV (P<0.05). Moreover, a lower blood pressure was observed with IPC compared to Sham (P<0.05). Prolonged sitting or IPC did not affect cerebrovascular responses (P>0.05). Collectively, these results indicate that the application of IPC prior to prolonged, uninterrupted sitting bout was associated with a better glucose tolerance and prevented impairment in (para)sympathetic nerve activity and blood pressure in healthy young men and women.

中文翻译：

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缺血预处理可防止长时间坐着对葡萄糖耐量和心血管健康标志物的影响，但不能防止脑血管反应。

长时间，不间断的坐姿可严重损害葡萄糖的体内稳态，但也会对心血管健康产生不利影响。我们检查了缺血预处理（IPC）是否能防止长时间坐姿诱发的葡萄糖耐量下降的影响，并测量了2h长时间坐姿期间的相关影响因素，如（交感神经）活动（通过心率变异性（HRV）评估）和血压。在这项随机，对照的交叉研究中，有15位健康参与者（80％的男性）的平均年龄为21±1岁（平均值±SD），体重指数为25.0±2.4 kg m ²进行IPC（IPC;大腿肌肉4×5-min 220-mmHg单侧闭塞）或假手术（Sham; 4×5-min 20-mmHg），然后坐2h。在IPC或Sham干预之后，在75 g葡萄糖摄入30、60、90和120分钟之前和之后测量指尖血糖。口服葡萄糖耐量试验期间的血糖反应显着减弱，导致坐位前先进行IPC搏动的曲线下方面积比假手术小（P <0.05）。IPC增加了HRV在120分钟时的高频振荡，并降低了低频振荡与高频振荡的比率（P <0.05）。此外，与假手术相比，IPC的血压更低（P <0.05）。长时间坐着或IPC不会影响脑血管反应（P> 0.05）。总的来说，