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Identification and iterative combinatorial mutagenesis of a new naringinase producting strain, Aspergillus tubingensis MN589840
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-11-08 , DOI: 10.1111/lam.13379 X-K Xia 1 , Y-E Zhang 1 , S-J Lei 1 , B Hu 1 , C-X Fu 2
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-11-08 , DOI: 10.1111/lam.13379 X-K Xia 1 , Y-E Zhang 1 , S-J Lei 1 , B Hu 1 , C-X Fu 2
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Naringinase was mainly obtained by microbial fermentation, and mutagenesis was a major way for obtaining excellent mutants. The aim of this study was to screen out a high naringinase yielding mutant to enhance the potential application value of its industrialization and compare the effects of different mutagenic methods on the enzyme activity of the strain. A novel producing naringinase strain, Aspergillus tubingensis MN589840, was isolated from mildewed pomelo peel, later subjected to mutagenesis including UV, ARTP and UV-ARTP. After five rounds iterative mutagenesis, the mutants U1, A6 and UA13 were screened out with 1448.49 U mg-1 , 1848.71 U mg-1 , 2475.16 U mg-1 enzyme activity, the naringinase productivity raised by 79.08%, 123.56% and 206%, respectively. In addition, the naringinase activity of three mutants rose after each round of iterative mutagenesis. These results indicated that the mutagenesis efficiency of UV-ARTP was higher than that of single ARTP, and both are better than UV. In summary, the iterative UV-ARTP mutagenesis is an effective strategy for screening high naringinase producing strains.
中文翻译：
柚皮苷酶新产菌株塔宾曲霉MN589840的鉴定及迭代组合诱变
柚皮苷酶主要通过微生物发酵获得，诱变是获得优良突变体的主要途径。本研究旨在筛选出一种柚苷酶高产突变体，以提高其产业化的潜在应用价值，并比较不同诱变方法对该菌株酶活性的影响。从发霉的柚皮中分离得到一株新的柚皮苷酶菌株——塔宾曲霉MN589840，经UV、ARTP和UV-ARTP诱变后分离得到。经过5轮迭代诱变，筛选出突变体U1、A6和UA13，酶活分别为1448.49 U mg-1、1848.71 U mg-1、2475.16 U mg-1，柚皮苷酶产率分别提高了79.08%、123.56%和123.56% ， 分别。此外，每轮迭代诱变后，三个突变体的柚皮苷酶活性均上升。这些结果表明UV-ARTP的诱变效率高于单一ARTP，且均优于UV。总之，迭代UV-ARTP诱变是筛选高产柚苷酶菌株的有效策略。
更新日期：2020-11-08
中文翻译：
柚皮苷酶新产菌株塔宾曲霉MN589840的鉴定及迭代组合诱变
柚皮苷酶主要通过微生物发酵获得，诱变是获得优良突变体的主要途径。本研究旨在筛选出一种柚苷酶高产突变体，以提高其产业化的潜在应用价值，并比较不同诱变方法对该菌株酶活性的影响。从发霉的柚皮中分离得到一株新的柚皮苷酶菌株——塔宾曲霉MN589840，经UV、ARTP和UV-ARTP诱变后分离得到。经过5轮迭代诱变，筛选出突变体U1、A6和UA13，酶活分别为1448.49 U mg-1、1848.71 U mg-1、2475.16 U mg-1，柚皮苷酶产率分别提高了79.08%、123.56%和123.56% ， 分别。此外，每轮迭代诱变后，三个突变体的柚皮苷酶活性均上升。这些结果表明UV-ARTP的诱变效率高于单一ARTP，且均优于UV。总之，迭代UV-ARTP诱变是筛选高产柚苷酶菌株的有效策略。
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