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Genotyping and antibiotic resistance patterns of Campylobacter fetus subsp . venerealis from cattle farms in India
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-09-16 , DOI: 10.1111/lam.13378 A Ishtifaq 1 , S Qureshi 1 , S Farooq 1 , Z A Kashoo 1 , Md Z Malik 2 , M R Alam 3 , S A Wani 1 , M A Bhat 1 , M I Hussain 1 , R A Dar 4 , S M Shah 5
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-09-16 , DOI: 10.1111/lam.13378 A Ishtifaq 1 , S Qureshi 1 , S Farooq 1 , Z A Kashoo 1 , Md Z Malik 2 , M R Alam 3 , S A Wani 1 , M A Bhat 1 , M I Hussain 1 , R A Dar 4 , S M Shah 5
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Bovine genital campylobacteriosis caused by Campylobacter fetus subsp. venerealis (Cfv) is of considerable economic importance to the cattle industry worldwide. Cfv causes syndrome of temporary infertility in female cattle, early embryonic mortality, aberrant oestrus cycles, delayed conception, abortions and poor calving rates. In the present study, a total of 200 samples obtained from vaginal swabs, cervicovaginal mucous (CVM), preputial washes and semen straws were investigated that were obtained from organized cattle farm of MLRI, Manasbal and unorganized sectors. Out of a total of 200 samples, 49 (47·57%) vaginal swabs, 1 (3·33%) preputial wash and 8 (25%) carried out CVM samples were positive for Cfv, whereas none of the semen straws were positive for Cfv. A total of eleven isolates of Cfv were recovered. PFGE (Pulse field gel electrophoresis) analysis revealed four different pulsotypes (I–IV) circulating in the screened farms. A common pulsotype circulating among farms could not be established. Insertion element (ISCfe1), a 233 bp amplicon of Cfv, was sequenced and the sequence was deposited in GenBank (accession no: MK475662).
中文翻译：
胎儿弯曲杆菌亚种的基因分型和抗生素耐药模式。来自印度养牛场的性病
由胎儿弯曲杆菌亚种引起的牛生殖器弯曲杆菌病。性病 (Cfv) 对全球养牛业具有相当重要的经济意义。Cfv 会导致雌性牛暂时性不孕症、胚胎早期死亡、发情周期异常、受孕延迟、流产和产犊率低。在本研究中，对从 MLRI、Manasbal 和无组织部门的有组织的养牛场获得的阴道拭子、宫颈阴道粘液 (CVM)、包皮冲洗液和精液吸管中获得的总共 200 个样本进行了调查。在总共 200 个样本中，49 个 (47·57%) 阴道拭子、1 个 (3·33%) 包皮清洗和 8 个 (25%) 进行的 CVM 样本对 Cfv 呈阳性，而精液吸管均未呈阳性对于 Cfv。共回收到 11 株 Cfv。PFGE（脉冲场凝胶电泳）分析揭示了在筛选农场中循环的四种不同的脉冲型（I-IV）。无法建立在农场之间循环的常见脉冲型。对插入元件 (ISCfe1)，Cfv 的 233 bp 扩增子进行测序，并将该序列保存在 GenBank 中（登录号：MK475662）。
更新日期：2020-09-16
中文翻译：
胎儿弯曲杆菌亚种的基因分型和抗生素耐药模式。来自印度养牛场的性病
由胎儿弯曲杆菌亚种引起的牛生殖器弯曲杆菌病。性病 (Cfv) 对全球养牛业具有相当重要的经济意义。Cfv 会导致雌性牛暂时性不孕症、胚胎早期死亡、发情周期异常、受孕延迟、流产和产犊率低。在本研究中，对从 MLRI、Manasbal 和无组织部门的有组织的养牛场获得的阴道拭子、宫颈阴道粘液 (CVM)、包皮冲洗液和精液吸管中获得的总共 200 个样本进行了调查。在总共 200 个样本中，49 个 (47·57%) 阴道拭子、1 个 (3·33%) 包皮清洗和 8 个 (25%) 进行的 CVM 样本对 Cfv 呈阳性，而精液吸管均未呈阳性对于 Cfv。共回收到 11 株 Cfv。PFGE（脉冲场凝胶电泳）分析揭示了在筛选农场中循环的四种不同的脉冲型（I-IV）。无法建立在农场之间循环的常见脉冲型。对插入元件 (ISCfe1)，Cfv 的 233 bp 扩增子进行测序，并将该序列保存在 GenBank 中（登录号：MK475662）。
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