Selaginella moellendorffii Hieron. (SM), a perennial evergreen plant, has been used in the treatment of acute infectious hepatitis, thoracic and hypochondriac lumbar contusions, systemic oedema and thrombocytopaenia. However, the role of a biflavonoid‐rich extract from SM (SM‐BFRE) in anti‐larynx cancer has rarely been reported. In this study, the in vitro and in vivo anti‐laryngeal cancer activity and potential mechanisms of SM‐BFRE were investigated. An off‐line semipreparative liquid chromatography‐nuclear magnetic resonance protocol was carried out to determine six biflavonoids from SM‐BFRE. In vitro, MTT assay revealed that SM‐BFRE inhibited the proliferation of laryngeal carcinoma cells. A wound healing assay indicated that SM‐BFRE suppressed the migration of laryngeal cancer cells. Hoechst 33 258 and Annexin V‐FITC/PI double staining assays were performed and verified that SM‐BFRE induced apoptosis in laryngeal carcinoma cells. The Hep‐2 bearing nude mouse model confirmed that SM‐BFRE also exhibited anticancer effect in vivo. In addition, Western blot analysis demonstrated that SM‐BFRE exerted its anti‐laryngeal cancer effect by activating the mitochondrial apoptotic pathway and inhibiting STAT3 and Akt/NF‐κB signalling pathways. All results suggested that SM‐BFRE could be considered as a potential chemotherapeutic drug for laryngeal cancer.

中文翻译：

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从卷柏中提取富含双黄酮的提取物。通过STAT3和Akt /NF-κB信号传导通路诱导喉癌细胞凋亡。

卷柏希龙 （SM）是一种多年生常绿植物，已用于治疗急性传染性肝炎，胸椎和软骨炎性腰椎挫伤，全身性水肿和血小板减少症。但是，很少有人报道SM中富含类黄酮的提取物（SM-BFRE）在抗喉癌​​中的作用。在这项研究中，研究了SM-BFRE的体外和体内抗喉癌活性和潜在机制。进行了离线半制备液相色谱-核磁共振方案，从SM-BFRE中测定了六种类黄酮。在体外，MTT分析显示SM-BFRE抑制了喉癌细胞的增殖。伤口愈合试验表明，SM-BFRE抑制了喉癌细胞的迁移。进行了Hoechst 33258和Annexin V‐FITC / PI双重染色测定，并验证了SM‐BFRE诱导喉癌细胞凋亡。带有Hep-2的裸鼠模型证实SM-BFRE在体内也表现出抗癌作用。此外，蛋白质印迹分析表明，SM‐BFRE通过激活线粒体凋亡途径并抑制STAT3和Akt / NF‐κB信号传导途径发挥其抗喉癌作用。所有结果表明，SM‐BFRE可被视为喉癌的一种潜在化疗药物。Western blot分析表明，SM‐BFRE通过激活线粒体凋亡途径并抑制STAT3和Akt / NF‐κB信号传导途径发挥抗喉癌作用。所有结果表明，SM‐BFRE可被视为喉癌的一种潜在化疗药物。Western blot分析表明，SM‐BFRE通过激活线粒体凋亡途径并抑制STAT3和Akt / NF‐κB信号传导途径发挥抗喉癌作用。所有结果表明，SM‐BFRE可被认为是喉癌的一种潜在化疗药物。