CO₂ toxicity, ionic strength, and specific culture media components, such as thiosulfate have been reported to influence the growth of CO₂-fixing autotrophic bacteria. In this study, a chemostat culture was used to determine the kinetics of CO₂ fixation by S. lithotrophicum 42BKT^T. The medium composition was determined to be capable of reaching a steady state at a high concentration of thiosulfate, an energy source, without growth inhibition by the excessively high ionic strength and thiosulfate itself. CO₂ was supplied by saturating the medium with a 1 atm CO₂ gas or a gaseous mixture of 0.8 atm N₂ and 0.2 atm CO₂. The Monod equation successfully described the kinetics of CO₂ fixation; the maximum specific growth rate was 0.33 h⁻¹ regardless of CO₂ pressure while the Monod constant was 0.0379 g Na₂S₂O₃/L and 0.196 g Na₂S₂O₃/L under 0.2 atm and 1.0 atm CO₂, respectively. We found that when nutrients, including thiosulfate, were supplied sufficiently, the CO₂ fixation rate at 1 atm CO₂ was only slightly lower than that at 0.2 atm CO₂ (e.g. only by 4.7 % for 3.0 g Na₂S₂O₃/L). Therefore, the CO₂ fixation by S. lithotrophicum 42BKT^T is not subject to CO₂ toxicity at least up to 1.0 atm CO₂.

据报道，CO ₂毒性，离子强度和特定的培养基成分（如硫代硫酸盐）会影响固定CO _2的自养细菌的生长。在这项研究中，使用了一种恒化器培养物来确定石蜡麦芽孢杆菌42BKT ^T对CO ₂固定的动力学。确定培养基组合物能够在高浓度的硫代硫酸盐（一种能量源）下达到稳态，而不会因过高的离子强度和硫代硫酸盐本身而抑制生长。CO ₂是由饱和用1个大气压的CO介质供给₂气体或0.8个大气压N A气态混合物₂个大气压的CO和0.2 ₂。Monod方程成功地描述了CO ₂固定的动力学。在0.2 atm和1.0 atm CO _2下，无论CO ₂压力如何，最大比生长速率均为0.33 h ^-1，而Monod常数为0.0379 g Na ₂ S ₂ O ₃ / L和0.196 g Na ₂ S ₂ O ₃ / L ，分别。我们发现，当提供足够的营养素（包括硫代硫酸盐）时，在1 atm CO ₂处的CO ₂固定率仅略低于在0.2 atm CO ₂处的固定率（例如，对于3.0 g Na ₂ S ₂仅降低4.7％O ₃ / L）。因此，CO ₂的固定由S. lithotrophicum 42BKT ^Ť不受CO ₂毒性至少达到1.0个大气压的CO ₂。