Abstract Xanthomonas citri subsp. citri (Xcc) is the important causal agent which causes citrus canker in citrus crops. In this study, a mutant strain with an in-frame deletion removed nucleotides 134 to 1017 of the vacJ gene was constructed to reveal the role of VacJ in the physiology and virulence from Xcc. Mutation of vacJ resulted in the decrease in biofilm formation ability in glass tubes and on leaf surfaces, swarming motility, bacterial growth in planta and virulence on the host plant mandarin orange compared with the wild-type strain. Our results show that the VacJ plays an important role in the virulence of X. citri subsp. citri that is associated with the regulation of biofilm formation and swarming motility. The results provided evidence that the lipoprotein VacJ plays an important role in the virulence of the plant pathogen X. citri subsp. citri.

中文翻译：

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脂蛋白 VacJ/MlaA 在植物病原体柑橘黄单胞菌的生理和毒力中的作用。柑橘

摘要柑橘黄单胞菌亚种。柑橘（Xcc）是导致柑橘类作物柑橘溃疡病的重要病原体。在这项研究中，构建了一个具有框内缺失删除 vacJ 基因 134 至 1017 位核苷酸的突变菌株，以揭示 VacJ 在 Xcc 的生理学和毒力中的作用。与野生型菌株相比，vacJ 的突变导致玻璃管和叶表面生物膜形成能力、蜂群运动性、植物细菌生长和对寄主植物橘子的毒力降低。我们的结果表明 VacJ 在 X. citri subsp. 的毒力中起重要作用。citri 与生物膜形成和蜂群运动的调节有关。结果证明脂蛋白 VacJ 在植物病原体 X 的毒力中起重要作用。柑橘亚种 柑橘。