Skeletal muscle, a significant contributor to resting energy expenditure and reactive oxygen species, may play critical role in body-weight regulation and aging processes. Methionine restriction (MR) is a dietary intervention which extends lifespan, lowers body-weight and enhances energy expenditure in rodents, all of which has been linked to mitochondrial function in various tissues including liver, kidney, heart and brown adipose tissue; however, mitochondrial responses to MR in skeletal muscle is largely unknown. Given the importance of skeletal muscle on energy mobilization and aging-related processes, we investigated if there are changes in skeletal muscle mitochondrial energetics in response to MR. Although MR lowers body-weight in rats, neither respiration, proton leak nor hydrogen peroxide metabolism was altered in isolated skeletal muscle mitochondria. This suggests that the function of skeletal muscle remains conserved while MR alters metabolism in other tissues.

中文翻译：

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短期蛋氨酸限制对 Fischer-344 大鼠骨骼肌线粒体过氧化氢代谢的影响

骨骼肌是静息能量消耗和活性氧的重要贡献者，可能在体重调节和衰老过程中发挥关键作用。限制蛋氨酸 (MR) 是一种饮食干预，可延长啮齿动物的寿命、降低体重并增加能量消耗，所有这些都与各种组织的线粒体功能有关，包括肝脏、肾脏、心脏和棕色脂肪组织；然而，骨骼肌中对 MR 的线粒体反应在很大程度上是未知的。鉴于骨骼肌对能量动员和衰老相关过程的重要性，我们调查了骨骼肌线粒体能量学是否存在响应 MR 的变化。尽管 MR 降低了大鼠的体重，但无论是呼吸，在分离的骨骼肌线粒体中，质子泄漏和过氧化氢代谢发生了改变。这表明骨骼肌的功能保持保守，而 MR 改变其他组织的代谢。