Background

Many studies have confirmed that “in situ ischemia postconditioning” (ISPostC) and “remote ischemic postconditioning” (RIPostC) can reduce cerebral ischemia/reperfusion injury, but there is no comparison was made on the consistency of neuroprotection in ISPostC and RIPostC to different ischemic duration and number of cycles.

New method

We used a transient middle cerebral artery occlusion model to compare the neuroprotection of ISPostC and RIPostC. We conducted ISPostC and RIPostC via brief and repeated MCA and Femoral artery occlusion followed by different ischemic duration and number of cycles. Infarct volume, brain edema, Neurological deficit scores and Apoptosis were evaluated.

Results

First, the ISPostC with three cycles of 10-s occlusion/30-s release of both carotid arteries and the RIPostC with three cycles of 10-min occlusion/10-min release of the left and right femoral arteries can obviously reduce cerebral infarction size, brain edema, apoptosis, and improve behavioral deficits than other approaches. Second, three cycles of ischemia/reperfusion may be the best for RIPostC.

Comparison with existing method(s)

In this paper, we compared different ischemic duration and frequency of ISPostC and RIPostC models to determine the best method. This conclusion helps to unify the experimental methods.

Conclusions

Different ischemic duration and frequency of ischemic postconditioning affect neuroprotection. three cycles of 10-s occlusion/30-s release of both carotid arteries and three cycles of 10-min occlusion/10-min release of both femoral arteries could be the first choice to study mechanisms of ischemic postconditioning and be conducive to the unification of research results.

中文翻译：

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不同的缺血持续时间和缺血后处理的频率会影响局灶性缺血性中风的神经保护。

背景

许多研究已经证实“原位缺血后处理”（ISPostC）和“远程缺血后处理”（RIPostC）可以减少脑缺血/再灌注损伤，但是没有对ISPostC和RIPostC中的神经保护与不同缺血的一致性进行比较。持续时间和周期数。

新方法

我们使用短暂的大脑中动脉闭塞模型来比较ISPostC和RIPostC的神经保护作用。我们通过短暂和反复的MCA和股动脉闭塞进行ISPostC和RIPostC，然后进行不同的缺血持续时间和周期数。评估梗死体积，脑水肿，神经功能缺损评分和细胞凋亡。

结果

首先，ISPostC闭塞三个动脉10s / 30s释放三个周期，RIPostC闭塞三个10min / 10min和左，右股动脉三个周期可以明显减少脑梗死面积，脑水肿，细胞凋亡，并比其他方法改善行为缺陷。其次，缺血/再灌注的三个周期对于RIPostC可能是最好的。

与现有方法的比较

在本文中，我们比较了ISPostC和RIPostC模型的不同缺血持续时间和频率，以确定最佳方法。这一结论有助于统一实验方法。

结论

不同的缺血持续时间和缺血后处理的频率会影响神经保护作用。颈动脉的10s闭塞/ 30s释放的三个周期和股动脉的10min闭塞/ 10min的释放的三个周期可能是研究缺血后适应机制的首选方法，有利于统一研究结果。