Two experiments were carried out to determine the apparent metabolizable energy, nitrogen-corrected apparent metabolizable energy (AMEn), and the standardized ileal amino acid digestibility (SIAAD) and apparent ileal amino acid digestibility of corn distillers dried grains with solubles (corn-DDGS), without or with the singular supplementation of protease and phytase in broiler diets. One hundred sixty-eight male Cobb 500 broilers (experiment 1) were nutrient-properly fed to 13 D of age. At 14 D of age, broilers were fed one of four experimental diets (reference diet, reference diet + corn-DDGS, reference diet + corn-DDGS with 200 mg/kg of protease, or that with 100 mg/kg of phytase for 10 D). There was an effect (P < 0.05) of phytase and protease supplementation on the metabolizable coefficients of dry matter and crude protein. There was no effect (P > 0.05) of the supplementation with protease and phytase on AMEn values. In experiment 2, one hundred sixty-eight male Cobb 500 broiler chickens were nutrient-adequately fed from 1 to 24 D of age. On D 24, broilers were allocated to 4 treatments (nitrogen-free diet [NFD], NFD + corn-DDGS, NFD with corn-DDGS with 200 mg/kg of protease, or that with 100 mg/kg of phytase for 5 D) in a completely randomized design. The SIAAD of all amino acids was increased (P < 0.01) by the addition of protease. Phytase supplementation also improved SIAAD of (P < 0.01) lysine, methionine, cystine, aspartic acid, glycine, serine, and proline of corn-DDGS. Therefore, it is recommended to supplement protease and phytase in diets for broilers formulated with corn-DDGS to increase amino acid digestibility.

进行了两个实验以确定表观可代谢能量，氮校正表观可代谢能量（AMEn）以及标准化的回肠氨基酸消化率（SIAAD）和表观回肠氨基酸消化率的可溶玉米酒干粮（corn-DDGS）在肉鸡日粮中不添加或不单独添加蛋白酶和植酸酶。对168名雄性Cobb 500雄性肉鸡（实验1）进行了适当的营养饲喂，使其年龄达到13D。在14日龄时，给肉鸡饲喂四种实验饮食中的一种（参考饮食，参考饮食+玉米DDGS，参考饮食+玉米DDGS，含200 mg / kg的蛋白酶，或含100 mg / kg的植酸酶，持续10天） D）。有影响（P <0.05）补充植酸酶和蛋白酶对干物质和粗蛋白的代谢系数。 补充蛋白酶和肌醇六磷酸酶对AMEn值没有影响（P > 0.05）。在实验2中，从1至24 D年龄对168只雄性Cobb 500肉鸡进行了营养充足的喂养。在第24天，将肉鸡分配到4种处理方法（无氮饮食[ NFD ]，NFD +玉米DDGS，含玉米DDGS和200 mg / kg蛋白酶的NFD或含100 mg / kg植酸酶的5d处理）。 ）完全随机设计。通过添加蛋白酶，所有氨基酸的SIAAD均增加（P  <0.01）。补充植酸酶还可以改善（P <0.01）玉米-DDGS的赖氨酸，蛋氨酸，胱氨酸，天冬氨酸，甘氨酸，丝氨酸和脯氨酸。因此，建议用玉米DDGS配制的肉鸡在日粮中补充蛋白酶和植酸酶，以增加氨基酸的消化率。