Abstract Nitrosomonas mobilis Ms1, a chemolithotrophic ammonia oxidizing bacterium, finds importance in wastewater treatment by virtue of its capability to oxidize ammonia into nitrite. However, its slow growth restricts its applicability in various bioremediation programmes. Response surface methodology with central composite design was employed to evaluate better growth yield (nitrification) of N. mobilis Ms1 with three culture medium variables, namely ammonia, sodium bicarbonate and pH. Enhanced nitrification was achieved with 6.36 mM NH3, 13.41 mM NaHCO3 and 8.8 pH. A general stoichiometric equation for redox reaction pertaining to biomass synthesis was formulated, and 0.012 g/L of biomass was estimated from the optimized medium. The optimized medium components were used to study the biofilm forming ability of N. mobilis Ms1. It was further characterized for its morphology and biochemical composition. Extra-polymeric substances (EPS) of the biofilm was found to be rich in protein. Qualitative analysis of fatty acid methyl ester (FAME) profile of suspended culture and biofilm reveals several saturated and unsaturated fatty acids. FAME consisting of C12:0 and C12:1 methyl esters can be regarded as the biomarkers of N.mobilis Ms1.

中文翻译：

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响应化学计量氨消耗的运动亚硝化单胞菌 Ms1 的浮游和生物膜培养的生长

摘要 运动亚硝化单胞菌 Ms1 是一种化学无机营养型氨氧化细菌，因其将氨氧化为亚硝酸盐的能力而在废水处理中具有重要意义。然而，它的缓慢增长限制了它在各种生物修复计划中的适用性。使用具有中心复合设计的响应面方法来评估具有三个培养基变量，即氨、碳酸氢钠和 pH 值的运动猪瘟 Ms1 更好的生长产量（硝化）。使用 6.36 mM NH3、13.41 mM NaHCO3 和 8.8 pH 实现了增强的硝化作用。制定了与生物质合成有关的氧化还原反应的一般化学计量方程，并从优化的培养基中估计了 0.012 g/L 的生物质。优化后的培养基成分用于研究运动链球菌 Ms1 的生物膜形成能力。对其形态和生化组成进行了进一步表征。发现生物膜的聚合物外物质 (EPS) 富含蛋白质。悬浮培养物和生物膜的脂肪酸甲酯 (FAME) 谱的定性分析揭示了几种饱和和不饱和脂肪酸。由 C12:0 和 C12:1 甲酯组成的 FAME 可以被认为是 N.mobilis Ms1 的生物标志物。