This work highlights microstructure and molecular vibration of mannosylerythritol lipids (MELs) from Pseudozyma aphidis B1 and Pseudozyma hubeiensis TS18 strains collected from brown algae and mangrove sediments. The scanning electron microscopy (SEM) shows the elongated structures with polar budding in the cells of B1 and TS18 yeast strains. The high-resolution transmission electron microscopy (HRTEM) identifies large lipid bodies that contain MELs confirmed by the anthrone test and thin layer chromatography. The HRTEM also reveals unknown electron dense inclusions. The surface-enhanced Raman scattering (SERS) was used to analysis molecular vibrations of cells, MEL mixtures, and purified MELs (A, B, and C) extracted from the B1 and TS18 cells. The peak analysis of Raman spectra suggests a higher level of saturation per fatty acid chain in MEL-B in both B1 and TS18 cells. This work demonstrates that the out-of-plane bending vibrations of the CH bonds in the range of 840−940 cm⁻¹ can serve an efficient indicator for detecting MEL-A, -B, and -C.

这项工作突出了蚜虫假单胞菌B1和湖北假单胞菌的甘露糖苷赤藓糖醇脂质（MELs）的微观结构和分子振动。从褐藻和红树林沉积物中收集的TS18菌株。扫描电子显微镜（SEM）显示了B1和TS18酵母菌株细胞中带有极性芽的细长结构。高分辨率透射电子显微镜（HRTEM）可以识别含有通过蒽酮测试和薄层色谱法确认的MEL的大脂质体。HRTEM还显示出未知的电子致密夹杂物。表面增强拉曼散射（SERS）用于分析细胞，MEL混合物以及从B1和TS18细胞中提取的纯化MEL（A，B和C）的分子振动。拉曼光谱的峰分析表明，B1和TS18细胞中MEL-B中每个脂肪酸链的饱和度较高。这项工作证明了C的平面外弯曲振动840-940 cm ^-1范围内的H键可以用作检测MEL-A，-B和-C的有效指标。