In this study, two metal Schiff base complexes (cobalt(II) and manganese(II), were synthesized and characterized by ¹H-NMR and FT-IR analyses. The in vitro binding behavior of this complexes with β-Casein (β-CN) was investigated by using biophysical techniques. For evaluation the thermodynamics parameters of interaction between Schiff base complexes and β-CN,the fluorescence data at different temperatures were done. The results showed that the intrinsic fluorescence of the β-CN was quenched by increasing the complexes through the dynamic quenching mechanism. Also, these complexes demonstrated a considrable binding affinity to β-CN and the process is mainly entropy driven. Fluorescence resonance energy transfer was used in order to estimating the distance between donor (β-CN) and acceptor (complex). Results demonstrated that the sequence of tendency of the complexes to β-CN was as follows: Mn-Schiff base complex > Co-Schiff base complex. All these experimental results propose that β-CN might act as carrier protein for Co(II) and Mn (II) complexes to deliver it to the target molecules.

在这项研究中，合成了两种金属席夫碱配合物（钴（II）和锰（II）），并用¹1 H-NMR和FT-IR分析。使用生物物理技术研究了该复合物与β-酪蛋白（β-CN）的体外结合行为。为了评估席夫碱配合物与β-CN相互作用的热力学参数，完成了不同温度下的荧光数据分析。结果表明，通过动态猝灭机理增加了配合物，β-CN的内在荧光被猝灭。而且，这些复合物显示出对β-CN的显着结合亲和力，并且该过程主要是由熵驱动的。为了估计供体（β-CN）和受体（复合物）之间的距离，使用了荧光共振能量转移。结果表明，该复合物趋于β-CN的趋势顺序为：Mn-席夫碱复合物> Co-席夫碱复合物。