Abstract

Experimental studies have been performed on Tetraselmis viridis (Rouchijajnen) R.E. Norris, Hori & Chihara (Chlorophyta) and Phaeodactylum tricornutum Bohlin (Bacillariophyta) cultures of Black Sea microalgae using TsV-S1 and PtV-S18 algal virus strains isolated from the Black Sea ecosystem. The study assesses the effects of light intensity and the initial abundance of microalgae cells on the onset of their infection by viruses and the decline in their abundance. As early as on the second or third day, the cells changed their shape and increased in volume in the cultures infected by viruses. At this time, a decrease was observed in chlorophyll a red autofluorescence, variable fluorescence per cell, and photochemical efficiency of photosystem 2. In the studied cultures, a reduction in cell abundance due to viral lysis was noted 1 to 2 days later. The threshold cell abundance at which this process was observed was independent of light conditions and amounted to 3 × 10⁵ cells/mL for T. viridis and 18 × 10⁵ cells/mL for Ph. tricornutum. The complete lysis of algae cells was detected by the end of the fourth to sixth day. In the dark, the effect of viruses on microalgae was insignificant.

中文翻译：

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病毒感染对黑海微藻四叶藻（Chlorophyta）和三角藻（Phaeodactylum tricornutum）（Bacillariophyta）的功能和溶解的影响

摘要

使用从黑海生态系统分离的TsV-S1和PtV-S18藻类病毒菌株，对黑海微藻的Tetraselmis viridis（Rouchijajnen）RE Norris，Hori＆Chihara（Chlorophyta）和Tricornutum tricornutum Bohlin（Bacillariophyta）培养物进行了实验研究。该研究评估了光强度和微藻细胞初始丰度对病毒感染的开始及其丰度下降的影响。早在第二天或第三天，在被病毒感染的培养物中，细胞就改变了形状并增加了体积。此时，观察到叶绿素a减少红色的自发荧光，每个细胞的可变荧光和光系统2的光化学效率。在研究的培养物中，在1至2天后注意到由于病毒裂解而导致的细胞丰度降低。观察了此过程在其中阈值细胞丰度是独立的光条件，达3×10 ⁵细胞/ mL为T.贻贝和18×10 ⁵个细胞/ mL的pH值。藻。在第四至第六天结束时检测到藻类细胞完全溶解。在黑暗中，病毒对微藻的影响微不足道。