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Hepatoprotective Angelica sinensis silver nanoformulation against multidrug resistant bacteria and the integration of a multicomponent logic gate system.
Nanoscale ( IF 5.8 ) Pub Date : 2020-08-31 , DOI: 10.1039/d0nr04744a Jouharsha Afthab 1 , Nafeesa Khatoon 1 , Lulu Zhou 1 , Tianming Yao 1 , Shuo Shi 1
Nanoscale ( IF 5.8 ) Pub Date : 2020-08-31 , DOI: 10.1039/d0nr04744a Jouharsha Afthab 1 , Nafeesa Khatoon 1 , Lulu Zhou 1 , Tianming Yao 1 , Shuo Shi 1
Affiliation
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The rampant usage of antibiotics has led to the emergence of toxicity, especially hepatotoxicity and the emergence of microbial drug resistance. Hence, a series of novel hepatoprotective, biocompatible, antibacterial silver nanoformulations (AS-AgNPs) were developed by using the important Chinese medicinal plant Angelica sinensis. The different size of AS-AgNPs were characterized by UV-Visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The size-dependent antibacterial properties of AS-AgNPs were investigated against Gram-positive, Gram-negative and multi-drug resistant bacteria. The minimum inhibitory concentration (MIC) of AS-AgNPs with different size against six bacteria was found to be in the range of 5–100 μg mL−1 with no resistance till 12 cycles. TEM and SEM results of bacteria after the treatment suggested that AS-AgNPs disrupted the cell membrane by creating pores. The cytocompatibility and cytoprotective effect of AS-AgNPs were evaluated against HepG2 cell lines, which showed that 85% of cells were viable up to 100 μg mL−1 of the concentration with almost no change in AST and ALT levels. Further, a logic combinatorial library, including basic logic gates (AND, OR, NOR, INHIBIT, IMPLICATION, and YES), three input logic gates (OR, and NOR) and combinatorial gates (INH-OR, INH-YES, INH-INH, AND-NOR, and NOT-AND-NOR) were designed by integrating multi-components based on the interaction between AS-AgNP1 and bacteria, where DiSC3(5) was used as the signal reporter. This system clearly demonstrates the ability of simple logic circuits to perform sophisticated analysis for the detection of multiple bacteria.
中文翻译：
保肝当归银纳米制剂对多药耐药细菌和多组分逻辑门系统的整合。
抗生素的猖usage使用导致了毒性的出现，尤其是肝毒性和微生物耐药性的出现。因此，利用重要的中草药当归开发了一系列新型的保护肝脏，生物相容性的抗菌银纳米制剂（AS-AgNPs）。。通过紫外可见光谱，透射电子显微镜（TEM），扫描电子显微镜（SEM），X射线衍射（XRD）和傅里叶变换红外光谱（FT-IR）表征了不同大小的AS-AgNPs。研究了AS-AgNPs对革兰氏阳性，革兰氏阴性和多药耐药细菌的大小依赖性抗菌特性。发现不同大小的AS-AgNP对6种细菌的最小抑菌浓度（MIC）在5–100μgmL -1范围内直到12个循环都没有阻力。处理后细菌的TEM和SEM结果表明，AS-AgNPs通过产生孔破坏了细胞膜。评估了AS-AgNPs对HepG2细胞系的细胞相容性和细胞保护作用，结果表明85％的细胞在浓度达100μgmL -1时都具有活力，而AST和ALT水平几乎没有变化。此外，逻辑组合库包括基本逻辑门（AND，OR，NOR，INHIBIT，IMPLICATION和YES），三个输入逻辑门（OR和NOR）和组合门（INH-OR，INH-YES，INH- INH，AND-NOR和NOT-AND-NOR）是根据AS-AgNP1与细菌（其中DiSC 3（5）被用作信号报告器。该系统清楚地展示了简单逻辑电路执行复杂分析以检测多种细菌的能力。
更新日期：2020-10-02
中文翻译：
保肝当归银纳米制剂对多药耐药细菌和多组分逻辑门系统的整合。
抗生素的猖usage使用导致了毒性的出现，尤其是肝毒性和微生物耐药性的出现。因此，利用重要的中草药当归开发了一系列新型的保护肝脏，生物相容性的抗菌银纳米制剂（AS-AgNPs）。。通过紫外可见光谱，透射电子显微镜（TEM），扫描电子显微镜（SEM），X射线衍射（XRD）和傅里叶变换红外光谱（FT-IR）表征了不同大小的AS-AgNPs。研究了AS-AgNPs对革兰氏阳性，革兰氏阴性和多药耐药细菌的大小依赖性抗菌特性。发现不同大小的AS-AgNP对6种细菌的最小抑菌浓度（MIC）在5–100μgmL -1范围内直到12个循环都没有阻力。处理后细菌的TEM和SEM结果表明，AS-AgNPs通过产生孔破坏了细胞膜。评估了AS-AgNPs对HepG2细胞系的细胞相容性和细胞保护作用，结果表明85％的细胞在浓度达100μgmL -1时都具有活力，而AST和ALT水平几乎没有变化。此外，逻辑组合库包括基本逻辑门（AND，OR，NOR，INHIBIT，IMPLICATION和YES），三个输入逻辑门（OR和NOR）和组合门（INH-OR，INH-YES，INH- INH，AND-NOR和NOT-AND-NOR）是根据AS-AgNP1与细菌（其中DiSC 3（5）被用作信号报告器。该系统清楚地展示了简单逻辑电路执行复杂分析以检测多种细菌的能力。
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