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Simple and reliable determination of Zn and some additional elements in seminal plasma samples by using total reflection X-ray fluorescence spectroscopy
Analytical Methods ( IF 2.7 ) Pub Date : 2020-08-31 , DOI: 10.1039/d0ay01185a Eva Marguí 1, 2, 3, 4 , Jerka Dumić 5, 6, 7, 8, 9 , Ignasi Queralt 4, 10, 11, 12 , Lada Baković 9, 13, 14, 15 , Jasna Jablan 5, 6, 8, 9, 16
Analytical Methods ( IF 2.7 ) Pub Date : 2020-08-31 , DOI: 10.1039/d0ay01185a Eva Marguí 1, 2, 3, 4 , Jerka Dumić 5, 6, 7, 8, 9 , Ignasi Queralt 4, 10, 11, 12 , Lada Baković 9, 13, 14, 15 , Jasna Jablan 5, 6, 8, 9, 16
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Trace elements are essential for the normal spermatogenesis of mammals and play a critical role in sperm quality and pathological processes e.g. inflammation. Consequently, multi-elemental analysis of seminal plasma (SP) may provide significant information on physiological and pathophysiological processes occurring in the male reproductive tract. Therefore, the development of a simple, fast and reliable method for seminal plasma (SP) analysis by total reflection X-ray fluorescence spectrometry (TXRF) could be useful for both, scientific and clinical studies. In this study, a detailed assessment of the sample preparation parameters and measurement conditions, including analysis of the shape and element distribution of the deposited residue on the reflector by micro X-ray fluorescence spectrometry, was carried out. Using the best analytical conditions, limits of detection for trace elements were found to be in the range of 0.04–0.3 mg kg−1. Trueness and precision of the results, evaluated by spiked SP sample analysis, were in most cases acceptable with recovery values in the range of 87–109% and relative standard deviations 3–12% (n = 5). The developed TXRF method was applied for the analysis of several SP samples from patients with different diagnoses and the results were compared with those obtained by ICP-OES. Among the studied trace elements with a role in the antioxidant defence system only Zn could be quantified and some differences in Zn concentrations among studied groups were observed. However, further studies on a large number of samples are required to define the exact relationship between the element composition and semen quality.
中文翻译：
使用全反射X射线荧光光谱法简单可靠地测定精浆中的锌和其他元素
微量元素对于哺乳动物的正常精子发生必不可少，并且在精子质量和病理过程中起着至关重要的作用，例如炎。因此，对精浆（SP）进行多元素分析可提供有关雄性生殖道中发生的生理和病理生理过程的重要信息。因此，开发一种通过全反射X射线荧光光谱（TXRF）分析精浆（SP）的简单，快速和可靠的方法，对于科学和临床研究都可能是有用的。在这项研究中，对样品制备参数和测量条件进行了详细的评估，包括通过微X射线荧光光谱法分析反射器上沉积的残留物的形状和元素分布。使用最佳分析条件，发现微量元素的检出限为0.04–0.3 mg kg -1。通过加标SP样品分析评估的结果的真实性和精确度在大多数情况下都可以接受，回收率范围为87–109％，相对标准偏差为3–12％（n = 5）。发达的TXRF方法用于分析来自不同诊断患者的几种SP样品，并将结果与ICP-OES获得的结果进行比较。在所研究的与抗氧化剂防御系统有关的微量元素中，仅锌可以定量，并且在所研究的组之间观察到锌浓度的一些差异。但是，需要对大量样品进行进一步研究，以定义元素组成与精液质量之间的确切关系。
更新日期：2020-09-25
中文翻译：
使用全反射X射线荧光光谱法简单可靠地测定精浆中的锌和其他元素
微量元素对于哺乳动物的正常精子发生必不可少，并且在精子质量和病理过程中起着至关重要的作用，例如炎。因此，对精浆（SP）进行多元素分析可提供有关雄性生殖道中发生的生理和病理生理过程的重要信息。因此，开发一种通过全反射X射线荧光光谱（TXRF）分析精浆（SP）的简单，快速和可靠的方法，对于科学和临床研究都可能是有用的。在这项研究中，对样品制备参数和测量条件进行了详细的评估，包括通过微X射线荧光光谱法分析反射器上沉积的残留物的形状和元素分布。使用最佳分析条件，发现微量元素的检出限为0.04–0.3 mg kg -1。通过加标SP样品分析评估的结果的真实性和精确度在大多数情况下都可以接受，回收率范围为87–109％，相对标准偏差为3–12％（n = 5）。发达的TXRF方法用于分析来自不同诊断患者的几种SP样品，并将结果与ICP-OES获得的结果进行比较。在所研究的与抗氧化剂防御系统有关的微量元素中，仅锌可以定量，并且在所研究的组之间观察到锌浓度的一些差异。但是，需要对大量样品进行进一步研究，以定义元素组成与精液质量之间的确切关系。
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