Sampling of prostate tissue (n = 97) was performed in conjunction with planned radical prostatectomies, in collaboration with Biobank1^®. The tissue used in this study was collected during the period 2003–2016, quickly frozen, and kept at −80°C until assayed in 2018. RNA extraction was performed with two different protocols (miRNeasy and mirVana™), and RNA quality was determined by measuring the RNA Integrity Number (RIN). The level of isoprostanes is widely recognized as a specific indicator of lipid peroxidation both in vitro and in vivo. The level of 8-isoprostane was measured because it is the main oxidation product of arachidonic acid, the most abundant phospholipid fatty acid. The level of 8-isoprostane was measured using enzyme immunoassay. There was no statistically significant difference in yield between the samples isolated with the mirVana protocol compared to the miRNeasy protocol. Average RIN was 2.8 units higher with the mirVana extraction protocol compared to the miRNeasy protocol (p < 0.001). For miRNeasy extractions, RINs were 7.1 for prostatectomies in 2005–2007 and 6.2 for those in 2018 (p < 0.001). For mirVana extractions, the difference in RIN score between the two groups regarding years of collection was not statistically significant. There was no significant increase in the levels of 8-isoprostane between the 2005–2007 samples and the 2018. The conclusion is that there is no oxidation of phospholipids with increasing storage time up to 15 years.

中文翻译：

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前列腺组织样本在 -80°C 下长期储存后，RNA 完整性和 8-异前列烷水平是稳定的

 与 Biobank1 ^®合作，结合计划的根治性前列腺切除术进行了前列腺组织取样 ( n = 97) 。本研究中使用的组织是在 2003-2016 年期间收集的，快速冷冻，并保存在 -80°C 直至 2018 年进行检测。RNA 提取采用两种不同的方案（miRNeasy 和mir Vana™）进行，RNA 质量为通过测量 RNA 完整性数 (RIN) 确定。异前列烷的电平被广泛认为是既脂质过氧化的特定指示符体外和体内. 测量 8-异前列烷的水平是因为它是花生四烯酸（最丰富的磷脂脂肪酸）的主要氧化产物。使用酶免疫测定法测量8-异前列烷的水平。与mir Vana 方案相比，使用mir Vana 方案分离的样品在产量上没有统计学上的显着差异。mir Vana 提取协议的平均 RIN比 mirNeasy 协议高 2.8 个单位( p  < 0.001)。对于 miRNeasy 提取，2005-2007 年前列腺切除术的 RIN 为 7.1，2018 年为 6.2（p  < 0.001）。对于米尔Vana 提取，两组之间 RIN 评分在收集年数方面的差异无统计学意义。2005-2007 年样品与 2018 年样品之间的 8-异前列烷水平没有显着增加。结论是，随着储存时间的增加，磷脂没有氧化，最多可达 15 年。