Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Histidine-Based Reduction-Sensitive Star-Polymer Inclusion Complex as a Potential DNA Carrier: Biophysical Studies Using Time-Resolved Fluorescence as an Important Tool.
Langmuir ( IF 3.7 ) Pub Date : 2020-08-31 , DOI: 10.1021/acs.langmuir.0c01636 Satyagopal Sahoo 1 , Sharmita Bera 1 , Dibakar Dhara 1
Langmuir ( IF 3.7 ) Pub Date : 2020-08-31 , DOI: 10.1021/acs.langmuir.0c01636 Satyagopal Sahoo 1 , Sharmita Bera 1 , Dibakar Dhara 1
Affiliation
|
An ideal DNA carrier is one that is capable of effectively condensing DNA into complexes of optimum size and shape, preventing premature decomplexation in the bloodstream and efficiently releasing the DNA into affected cells. In this context, we have developed a novel β-cyclodextrin (β-CD)-based four-arm star-shaped polymer inclusion complex (IC) with arms made of a poly(l-histidine)-based cationic polymer. The polymer was well characterized by gel permeation chromatography, NMR, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. We have also investigated its DNA complexation and release properties. Bisadamantane containing a disulfide bond was synthesized that linked two such poly(l-histidine)-containing β-CD units via guest–host interactions to prepare the presented IC. Besides using the conventional steady-state fluorescence spectroscopy, the ability of this IC to condense DNA to form polyplexes and their release behavior have been established by using the time-resolved fluorescence spectroscopy technique. Thiazole orange (TO) was used for the first time as a DNA-intercalating dye in the time-resolved fluorescence spectroscopic study. The superior DNA-condensing ability of the IC as compared to that of the precursor two-arm β-CD and linear poly(l-histidine) of a comparable molecular weight, as confirmed by dynamic light scattering, zeta potential, atomic force microscopy, and gel electrophoresis studies, could be attributed to a higher charge density. The IC–DNA polyplexes were found to be stable in a medium similar to an extracellular fluid but could efficiently release DNA in the presence of 10 mM glutathione, a concentration prevalent in the intracellular fluid of cancer cells. Hence, here, we have successfully demonstrated the synthesis of a novel biocompatible star-shaped IC with the potential to carry and release DNA in cancer cells and also established the feasibility of using the time-resolved fluorescence spectroscopic technique to study the complexation behavior of the polycation and DNA using TO as a DNA-intercalating dye.
中文翻译：
基于组氨酸的还原敏感型星形聚合物包合物作为潜在的DNA载体：使用时间分辨荧光作为重要工具的生物物理研究。
理想的DNA载体是能够有效地将DNA浓缩成最佳大小和形状的复合物，防止血液中过早分解并有效地将DNA释放到受影响细胞中的载体。在此背景下，我们开发了一种新型的基于β-环糊精（β-CD）的四臂星形聚合物包合物（IC），其臂由基于聚（1-组氨酸）的阳离子聚合物制成。通过凝胶渗透色谱，NMR和基质辅助激光解吸电离飞行时间质谱对聚合物进行了很好的表征。我们还研究了其DNA络合和释放特性。合成了含有二硫键的双丹丹烷，将两个这样的聚（l-组氨酸）通过来宾-宿主相互作用制备β-CD单元，以制备本文提出的IC。除了使用常规的稳态荧光光谱法外，还通过使用时间分辨荧光光谱技术确定了该IC凝聚DNA形成多链体的能力及其释放行为。在时间分辨荧光光谱研究中，噻唑橙（TO）首次用作DNA嵌入染料。与前者的两臂β-CD和线性聚（l动态光散射，ζ电位，原子力显微镜和凝胶电泳研究证实，具有相当分子量的α-组氨酸）可归因于较高的电荷密度。发现IC–DNA多聚体在类似于细胞外液的培养基中稳定，但在10 mM谷胱甘肽（一种在癌细胞的细胞内液中普遍存在的浓度）存在的情况下，可以有效释放DNA。因此，在这里，我们已经成功地证明了新型的具有生物相容性的星形IC的合成，它具有在癌细胞中携带和释放DNA的潜力，并且还确立了使用时间分辨荧光光谱技术来研究该化合物的络合行为的可行性。聚阳离子和DNA，使用TO作为DNA嵌入染料。
更新日期：2020-09-29
中文翻译：
基于组氨酸的还原敏感型星形聚合物包合物作为潜在的DNA载体：使用时间分辨荧光作为重要工具的生物物理研究。
理想的DNA载体是能够有效地将DNA浓缩成最佳大小和形状的复合物，防止血液中过早分解并有效地将DNA释放到受影响细胞中的载体。在此背景下，我们开发了一种新型的基于β-环糊精（β-CD）的四臂星形聚合物包合物（IC），其臂由基于聚（1-组氨酸）的阳离子聚合物制成。通过凝胶渗透色谱，NMR和基质辅助激光解吸电离飞行时间质谱对聚合物进行了很好的表征。我们还研究了其DNA络合和释放特性。合成了含有二硫键的双丹丹烷，将两个这样的聚（l-组氨酸）通过来宾-宿主相互作用制备β-CD单元，以制备本文提出的IC。除了使用常规的稳态荧光光谱法外，还通过使用时间分辨荧光光谱技术确定了该IC凝聚DNA形成多链体的能力及其释放行为。在时间分辨荧光光谱研究中，噻唑橙（TO）首次用作DNA嵌入染料。与前者的两臂β-CD和线性聚（l动态光散射，ζ电位，原子力显微镜和凝胶电泳研究证实，具有相当分子量的α-组氨酸）可归因于较高的电荷密度。发现IC–DNA多聚体在类似于细胞外液的培养基中稳定，但在10 mM谷胱甘肽（一种在癌细胞的细胞内液中普遍存在的浓度）存在的情况下，可以有效释放DNA。因此，在这里，我们已经成功地证明了新型的具有生物相容性的星形IC的合成，它具有在癌细胞中携带和释放DNA的潜力，并且还确立了使用时间分辨荧光光谱技术来研究该化合物的络合行为的可行性。聚阳离子和DNA，使用TO作为DNA嵌入染料。
京公网安备 11010802027423号