Abstract The main objective of this study is to obtain some knowledge of cationic and anionic radicals in early embryo implantation in mice. The method used in this study is known as histochemical staining, in which Xylidine ponceau was used at pH 2.5 and toluidine blue was used at pH 4.0. We detected the change in glycosaminoglycans and total proteins in the endometrial stroma during the preimplantation of mice. This study revealed that the distribution patterns of cationic radicals and anionic radicals are similar on days 4 and 5 of pregnancy. However, there was a distinct difference between cationic radicals and anionic radicals on day 8 of pregnancy. The distribution pattern of cationic radicals is more concentrated in the stroma near the conceptus. Laboratory studies on histochemical stain provide more information about early embryo implantation.

中文翻译：

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胚胎植入早期阳离子和阴离子自由基的时空分布

摘要 本研究的主要目的是了解小鼠早期胚胎植入中阳离子和阴离子自由基的一些知识。本研究中使用的方法被称为组织化学染色，其中在 pH 2.5 下使用二甲苯胺丽红，在 pH 4.0 下使用甲苯胺蓝。我们检测了小鼠胚胎植入前子宫内膜基质中糖胺聚糖和总蛋白的变化。该研究表明，阳离子自由基和阴离子自由基在怀孕第 4 天和第 5 天的分布模式相似。然而，怀孕第8天的阳离子自由基和阴离子自由基之间存在明显差异。阳离子基团的分布模式更集中在胎体附近的基质中。