Abstract

The selected six specific bacterial and fungal strains, which caused oral teeth decay were analyzed by antimicrobial iron oxide (Fe₂O₃), copper oxide (Cu₄O₃), and zinc oxide (ZnO) nanoparticles synthesized by aloe barbadensis leaves extract, using agar well diffusion method with 0.9% saline solution. TMOs comparatively had not been focused on oral cavity infectious pathogens to analyze antimicrobial activity. In the present study, TMOs were characterized by UV, SEM, FTIR, and XRD. Oral infectious strains (Escherichia coli, Bacillus subtilis, Bacillus licheniformis, Klebsiella pneumonia) and fungal strains (Aspergillus niger, Candida albicans) were used as test microorganisms. The effect of different concentrations of TMO-NPs (2.5 µg/mL, 5 µg/mL, 10 µg/mL) was studied by zone of inhibition (ZOI), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The zinc oxide nanoparticle with least particle-sized (37.8 nm) exhibited highest antimicrobial activity. In future, these zinc-oxide nanoparticles may be used in medicine for oral infectious diseases treatment.

摘要

通过芦荟叶提取物合成的抗菌氧化铁（Fe ₂ O ₃），氧化铜（Cu ₄ O ₃）和氧化锌（ZnO）纳米颗粒分析了导致口腔龋齿的六种特定细菌和真菌菌株，采用琼脂井扩散法，加0.9％盐水溶液。相对而言，TMO尚未集中在口腔感染性病原体上以分析抗菌活性。在本研究中，通过UV，SEM，FTIR和XRD对TMO进行了表征。口腔感染菌株（大肠杆菌，枯草芽孢杆菌，地衣芽孢杆菌，肺炎克雷伯菌）和真菌菌株（黑曲霉，白色念珠菌）用作测试微生物。通过抑制区（ZOI），最小抑菌浓度（MIC）和最小杀菌浓度（MBC）研究了不同浓度的TMO-NPs（2.5 µg / mL，5 µg / mL，10 µg / mL）的影响。具有最小粒径（37.8nm）的氧化锌纳米颗粒表现出最高的抗菌活性。将来，这些氧化锌纳米粒子可用于治疗口腔传染病的药物。