当前位置: X-MOL 学术Prep. Biochem. Biotechnol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Rapid extraction of watermelon seed proteins using microwave and its functional properties
Preparative Biochemistry & Biotechnology ( IF 2.0 ) Pub Date : 2020-08-31 , DOI: 10.1080/10826068.2020.1808792
Manali Behere 1 , Sujata S Patil 1 , Virendra K Rathod 1
Affiliation  

Abstract

Solid food industry waste like watermelon seed is an excellent source of value-added components such as proteins, oil, and carbohydrate. In the present study, protein extraction was carried out using microwave energy from defatted watermelon seeds (DWS), containing 50% of proteins. Microwave-assisted extraction (MAE) was optimized with different parameters, namely, solid to solvent ratio (1:10–1:40), pH (7–10), microwave power (30 W, 50 W, 70 W), extraction time (30 s–8 min) and moisture content or pre-leaching effect. Maximum protein recovery was achieved with 50 W microwave power, solid to solvent ration of 1:30, and pH 10 in 2 minutes of microwave irradiation time. MAE gave higher yield in less time compared to conventional extraction. SDS-PAGE confirmed the molecular weight of watermelon seed proteins (WSP) in the range of 25–250 kDa. A comparative study showed 90% protein recovery with MAE in 2 min with 1:30 (w/v) solid to solvent ratio, whereas ultrasound gave 87% in 9 min with 1:50 (w/v) ratio and batch 72% in 25 min with 1:70 (w/v) ratio. Watermelon seed proteins obtained from MAE method possess excellent functional properties with reference to conventional extraction method indicating its application in food products.



中文翻译:

微波快速提取西瓜种子蛋白及其功能特性

抽象的

诸如西瓜籽之类的固体食品工业废物是诸如蛋白质,油脂和碳水化合物等增值成分的极佳来源。在本研究中,蛋白质的提取是利用微波能量从含有50%蛋白质的脱脂西瓜种子(DWS)中提取的。微波辅助萃取(MAE)使用不同的参数进行了优化,即固液比(1:10-1:40),pH(7-10),微波功率(30 W,50 W,70 W),萃取时间(30 s–8分钟)和水分含量或预浸效果。在微波辐射时间2分钟内,使用50 W微波功率,固体对溶剂的比例为1:30,pH为10可获得最大的蛋白质回收率。与常规提取相比,MAE在较短的时间内提供了更高的产量。SDS-PAGE证实了西瓜种子蛋白(WSP)的分子量在25–250 kDa的范围内。一项比较研究表明,MAE在2分钟内以1:30(w / v)的固溶比回收90%的蛋白质,而超声在9分钟内以1:50(w / v)的比率回收了87%,而批次在72分钟内的回收率为72%。 25分钟,以1:70(w / v)比率进行。MAE法制得的西瓜种子蛋白具有优良的功能特性,相对于传统的提取方法,表明其在食品中的应用。

更新日期:2020-08-31
down
wechat
bug