Cecropia pachystachya

 leaves are popularly used to treat asthma and diabetes. Despite the widespread consumption of this plant, there are few scientific studies regarding its toxicological potential. In order to conduct a thorough study concerning the potential adverse effects, the aim of this study was to assess acute and subacute toxicity tests of crude aqueous extract from C. pachystachya leaves (CAE-Cp) using in vivomodel, as well as in vitro cytotoxicity, genotoxicity and antioxidant activity. In addition, genotoxicity, and cytotoxicity of chlorogenic acid (CGA) and cytotoxicity of isoorientin (ISOO) were also evaluated. The antioxidant activity was verified by DPPH, cytotoxicity using sulforhodamine B (SRB) assay and genotoxicity by comet assay on V79 cells. The phytochemical analysis of CAE-Cp detected flavonoids and tannins, CGA and ISOO as the major compounds utilizing HPLC. The total flavonoid content (6.52 mg/g EQ) and antioxidant activity (EC₅₀ = 62.15 µg/ml) of CAE-Cp were determined. In vitro evaluations with CAE-Cp showed genotoxic effects at 0.31 to 2.5 mg/ml and an expressive cytotoxicity on HT-29 (IC₅₀ = 4.43 µg/ml) cells. CGA was genotoxic against V79 cells at 0.07 mg/ml and cytotoxic against to HT-29 (IC₅₀ = 71.70 µg/ml), OVCAR-3 (IC₅₀ = 80.07 µg/ml), MCF-7 (IC₅₀ = 45.58 µg/ml) and, NCI-H460 (IC₅₀ = 71.89 µg/ml) cancer cell lines. Wistar rats treated with a single dose (2,000 mg/kg) CAE-Cp decreased hemoglobin levels after 14 days, although no significant toxicity was observed in animals after 28 days. In view of the in vitro cytotoxicity and genotoxicity detected, further studies are necessary to establish the safe use of CAE-Cp.

中文翻译：

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柏柏叶的水提取物的体内和体外毒理学评价。

头孢菌属

 叶子通常用于治疗哮喘和糖尿病。尽管这种植物被广泛消费，但是关于其毒理学潜力的科学研究却很少。为了进行有关的潜在不利影响了深入的研究，本研究的目的是评估从粗水提取物的急性和亚急性毒性试验C. pachystachya使用叶（CAE-CP）在体内模型中，以及在体外细胞毒性，遗传毒性和抗氧化活性。此外，还评估了绿原酸（CGA）的遗传毒性和细胞毒性以及异Orientinin（ISOO）的细胞毒性。通过DPPH，使用磺基罗丹明B（SRB）测定的细胞毒性和通过彗星测定对V79细胞的遗传毒性验证了抗氧化活性。CAE-Cp的植物化学分析利用HPLC测定了类黄酮和单宁，CGA和ISOO为主要化合物。 测定了CAE-Cp的总黄酮含量（6.52 mg / g EQ）和抗氧化活性（EC ₅₀ = 62.15 µg / ml）。用CAE-Cp进行的体外评估显示了0.31至2.5 mg / ml的遗传毒性作用，并且对HT-29具有表达性的细胞毒性（IC ₅₀  = 4.43 µg / ml）细胞。CGA对V79细胞有0.07 mg / ml的遗传毒性，对HT-29（IC ₅₀  = 71.70  µg / ml），OVCAR-3（IC ₅₀  = 80.07  µg / ml），MCF-7（IC ₅₀  = 45.58  µg ）具有细胞毒性。/ ml）和NCI-H460（IC ₅₀  = 71.89 µg / ml）癌细胞系。Wistar大鼠用单剂量（2,000 mg / kg）CAE-Cp处理后14天后血红蛋白水平降低，尽管在28天后未观察到明显的毒性。鉴于检测到的体外细胞毒性和基因毒性，需要进一步的研究来确定CAE-Cp的安全使用。