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Fc Receptor is Involved in Nk Cell Functional Anergy Induced by Miapaca2 Tumor Cell Line.
Immunological Investigations ( IF 2.9 ) Pub Date : 2020-08-30 , DOI: 10.1080/08820139.2020.1813757
Yekaterina O Ostapchuk 1 , Yuliya V Perfilyeva 1 , Aikyn Kali 1, 2 , Raikhan Tleulieva 1 , Oxana Yu Yurikova 1 , Gulshan E Stanbekova 3 , Boris V Karalnik 4 , Nikolai N Belyaev 5
Affiliation  

Impaired NK cytotoxicity has been linked to poor cancer prognosis, but its mechanisms are not clearly established. Increasing data demonstrate that NK cells lose cytotoxicity after interaction with NK cell-sensitive tumor cells. In this paper, we provide evidence that the human adenocarcinoma cell line MiaPaCa2 and TNFα and TGFβ-treated MiaPaCa2 cultures (MiaPaCa2-TT) induced functional anergy of NK cells via FGL2 protein. MiaPaCa2-TT cultures decreased expression of IFNγ, CD107a, DNAM-1, and stimulated expression of PD1 by NK cells, as well as inhibited their cytotoxic activity in a greater manner compared to the parental culture. More importantly, we found that co-cultivation with anergized NK cells decreased expression of IFNγ and CD107a by naïve NK cells, which supports the hypothesis of NK cell functional anergy transmission. The obtained results suggest a mechanism by which tumor cells may inhibit cytotoxic functions of tumor-infiltrating and circulating NK cells in cancer.

Abbreviations

CFSE: Carboxyfluorescein diacetate succinimidyl ester; CSCs: Cancer stem cells; FGL2: Fibrinogen-like protein 2; mAbs: Monoclonal antibodies; MiaPaCa2: Human adenocarcinoma cell line; MiaPaCa2-ТТ: Adenocarcinoma cell line MiaPaCa2 cells stimulated with TNFα and TGFβ-1; PI: Propidium iodide; TGFβ: Transforming growth factor beta; TME: Tumor microenvironment; TNFα: Tumor necrosis factor alfa



中文翻译:

Fc 受体参与 Miapaca2 肿瘤细胞系诱导的 Nk 细胞功能性无反应。

NK细胞毒性受损与癌症预后不良有关,但其机制尚不清楚。越来越多的数据表明,NK 细胞在与 NK 细胞敏感的肿瘤细胞相互作用后会失去细胞毒性。在本文中,我们提供证据表明人腺癌细胞系 MiaPaCa2 和 TNFα 和 TGFβ 处理的 MiaPaCa2 培养物 (MiaPaCa2-TT)通过以下途径诱导 NK 细胞的功能性无反应。FGL2 蛋白。与亲本培养相比,MiaPaCa2-TT 培养物降低了 IFNγ、CD107a、DNAM-1 的表达,并刺激了 NK 细胞的 PD1 表达,并以更大的方式抑制了它们的细胞毒活性。更重要的是,我们发现与失能的 NK 细胞共培养降低了幼稚 NK 细胞的 IFNγ 和 CD107a 的表达,这支持了 NK 细胞功能性无能传递的假设。获得的结果表明肿瘤细胞可能抑制癌症中肿瘤浸润和循环 NK 细胞的细胞毒性功能的机制。

缩写

CFSE:羧基荧光素二乙酸琥珀酰亚胺酯;CSCs:癌症干细胞;FGL2:纤维蛋白原样蛋白 2;mAbs:单克隆抗体;MiaPaCa2:人腺癌细胞系;MiaPaCa2-ТТ:用 TNFα 和 TGFβ-1 刺激的腺癌细胞系 MiaPaCa2 细胞;PI:碘化丙啶;TGFβ:转化生长因子β;TME:肿瘤微环境;TNFα:肿瘤坏死因子α

更新日期:2020-08-31
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