Transcriptomic similarities and differences between the limb bud AER and unique carapacial ridge of turtle embryos.,Evolution and Development

当前位置： X-MOL 学术 › Evol. Dev. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Transcriptomic similarities and differences between the limb bud AER and unique carapacial ridge of turtle embryos.
Evolution and Development ( IF 2.6 ) Pub Date : 2020-08-30 , DOI: 10.1111/ede.12351
Gerardo A Cordero ₁

Affiliation

Evolutionary innovation may arise via major departures from an ancestral condition. Turtle shell morphogenesis depends on a unique structure known as the carapacial ridge (CR). This lateral tissue protrusion in turtle embryos exhibits similar properties as the apical ectodermal ridge (AER)—a well‐known molecular signaling center involved in limb development. Still, how the CR influences shell morphogenesis is not entirely clear. The present study aimed to describe the CR transcriptome shortly before ribs were halted within its mesenchyme, as required for shell development. Analyses exposed that the mesenchymal marker VIM was one of the most highly co‐expressed genes and numerous appendage formation genes were situated within the core of CR and AER co‐expression networks. However, there were tissue‐specific differences in the activity of these genes. For instance, WNT5A was most frequently assigned to appendage‐related annotations of the CR network core, but not in the AER. Several homeobox transcription factors known to regulate limb bud patterning exhibited their highest expression levels in the AER, but were underexpressed in the CR. The results of this study corroborate that novel body plans often originate via alterations of pre‐existing genetic networks. Altogether, this exploratory study enhances the groundwork for future experiments on the molecular underpinnings of turtle shell development and evolution.

中文翻译：

肢芽AER和龟胚独特的甲状突脊之间的转录组学相似性和差异。

进化创新可能是通过与祖先条件的重大背离而产生的。龟壳的形态发生取决于一个独特的结构，称为甲壳ridge（CR）。龟胚中的这种侧向组织突出表现出与顶皮外皮（AER）相似的特性，顶皮外皮dermal是参与肢体发育的著名分子信号中心。尽管如此，CR如何影响壳的形态发生还不是很清楚。本研究旨在描述在肋骨间质停止之前不久的CR转录组，这是壳发育所必需的。分析表明，间充质标记物VIM是最高度共表达的基因之一，许多附属物形成基因位于CR和AER共表达网络的核心内。然而，这些基因的活性存在组织特异性差异。例如，WNT5A最常分配给CR网络核心的与附件相关的注释，而不是AER。几种已知的能调节肢芽模式的同源盒转录因子在AER中表现出最高的表达水平，但在CR中却表达不足。这项研究的结果证实了新颖的人体计划通常是通过改变现有的遗传网络而产生的。总而言之，这项探索性研究为今后有关龟壳发育和进化的分子基础的实验奠定了基础。几种已知的能调节肢芽模式的同源盒转录因子在AER中表现出最高的表达水平，但在CR中却表达不足。这项研究的结果证实了新的身体计划通常是通过改变现有的遗传网络而产生的。总之，这项探索性研究为乌龟壳发育和进化的分子基础的未来实验奠定了基础。几种已知的调节盒芽构型的同源盒转录因子在AER中表现出最高的表达水平，但在CR中却表达不足。这项研究的结果证实了新的身体计划通常是通过改变现有的遗传网络而产生的。总之，这项探索性研究为乌龟壳发育和进化的分子基础的未来实验奠定了基础。

更新日期：2020-09-28

点击分享查看原文

点击收藏

阅读更多本刊最新论文