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Bacillus subtilis as a host for mosquitocidal toxins production.
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-08-30 , DOI: 10.1111/1751-7915.13648 Emanuela Ursino 1 , Alessandra M Albertini 1 , Giulia Fiorentino 1 , Paolo Gabrieli 1 , Viola Camilla Scoffone 1 , Angelica Pellegrini 1 , Giuliano Gasperi 1 , Alessandro Di Cosimo 1 , Giulia Barbieri 1
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-08-30 , DOI: 10.1111/1751-7915.13648 Emanuela Ursino 1 , Alessandra M Albertini 1 , Giulia Fiorentino 1 , Paolo Gabrieli 1 , Viola Camilla Scoffone 1 , Angelica Pellegrini 1 , Giuliano Gasperi 1 , Alessandro Di Cosimo 1 , Giulia Barbieri 1
Affiliation
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Aedes albopictus transmits several arboviral infections. In the absence of vaccines, control of mosquito populations is the only strategy to prevent vector‐borne diseases. As part of the search for novel, biological and environmentally friendly strategies for vector control, the isolation of new bacterial species with mosquitocidal activity represents a promising approach. However, new bacterial isolates may be difficult to grow and genetically manipulate. To overcome these limits, here we set up a system allowing the expression of mosquitocidal bacterial toxins in the well‐known genetic background of Bacillus subtilis. As a proof of this concept, the ability of B. subtilis to express individual or combinations of toxins of Bacillus thuringiensis israelensis (Bti) was studied. Different expression systems in which toxin gene expression was driven by IPTG‐inducible, auto‐inducible or toxin gene‐specific promoters were developed. The larvicidal activity of the resulting B. subtilis strains against second‐instar Ae. albopictus larvae allowed studying the activity of individual toxins or the synergistic interaction among Cry and Cyt toxins. The expression systems here presented lay the foundation for a better improved system to be used in the future to characterize the larvicidal activity of toxin genes from new environmental isolates.
中文翻译：
枯草芽孢杆菌作为产生杀蚊毒素的宿主。
白纹伊蚊传播多种虫媒病毒感染。在没有疫苗的情况下，控制蚊子数量是预防媒介传播疾病的唯一策略。作为寻找新颖、生物和环境友好的病媒控制策略的一部分，分离具有杀蚊活性的新细菌物种是一种有前途的方法。然而，新的细菌分离株可能难以生长和基因操纵。为了克服这些限制，我们在这里建立了一个系统，允许在众所周知的枯草芽孢杆菌遗传背景中表达杀蚊细菌毒素。作为这一概念的证明，研究了枯草芽孢杆菌表达以色列苏云金芽孢杆菌（Bti）的单个毒素或毒素组合的能力。开发了不同的表达系统，其中毒素基因表达由 IPTG 诱导型、自诱导型或毒素基因特异性启动子驱动。所得枯草芽孢杆菌菌株对二龄伊蚊的杀幼虫活性。白纹伊蚊幼虫可以研究单个毒素的活性或 Cry 和 Cyt 毒素之间的协同相互作用。这里提出的表达系统为将来用于表征新环境分离株毒素基因的杀幼虫活性的更好改进系统奠定了基础。
更新日期：2020-10-05
中文翻译：
枯草芽孢杆菌作为产生杀蚊毒素的宿主。
白纹伊蚊传播多种虫媒病毒感染。在没有疫苗的情况下，控制蚊子数量是预防媒介传播疾病的唯一策略。作为寻找新颖、生物和环境友好的病媒控制策略的一部分，分离具有杀蚊活性的新细菌物种是一种有前途的方法。然而，新的细菌分离株可能难以生长和基因操纵。为了克服这些限制，我们在这里建立了一个系统，允许在众所周知的枯草芽孢杆菌遗传背景中表达杀蚊细菌毒素。作为这一概念的证明，研究了枯草芽孢杆菌表达以色列苏云金芽孢杆菌（Bti）的单个毒素或毒素组合的能力。开发了不同的表达系统，其中毒素基因表达由 IPTG 诱导型、自诱导型或毒素基因特异性启动子驱动。所得枯草芽孢杆菌菌株对二龄伊蚊的杀幼虫活性。白纹伊蚊幼虫可以研究单个毒素的活性或 Cry 和 Cyt 毒素之间的协同相互作用。这里提出的表达系统为将来用于表征新环境分离株毒素基因的杀幼虫活性的更好改进系统奠定了基础。
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