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Detection of Living Cervical Cancer Cells by Transient Terahertz Spectroscopy.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-08-30 , DOI: 10.1002/jbio.202000237 Wei Shi 1 , Yuezheng Wang 1 , Lei Hou 1 , Cheng Ma 1 , Lei Yang 1 , Chengang Dong 1 , Zhiquan Wang 1 , Haiqing Wang 1 , Juan Guo 2 , Shenglong Xu 2 , Jing Li 2
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2020-08-30 , DOI: 10.1002/jbio.202000237 Wei Shi 1 , Yuezheng Wang 1 , Lei Hou 1 , Cheng Ma 1 , Lei Yang 1 , Chengang Dong 1 , Zhiquan Wang 1 , Haiqing Wang 1 , Juan Guo 2 , Shenglong Xu 2 , Jing Li 2
Affiliation
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The photonic energy of terahertz wave is in the same order of magnitude as the rotational and vibrational energy levels of organic and biological macromolecules, so it has unique advantages in detecting cells and biological macromolecules. However, in the life environment, the dynamic time scale of cell‐environment interaction and structural conformation change of biological macromolecules are within picosecond to millisecond, and water has strong absorption to terahertz wave, which has become the bottleneck problem for the detection of cells and biological macromolecules by terahertz technology. In this article, we developed a set of terahertz single measurement system based on the tilt wave front of grating pulse technique. The system was employed for the terahertz detection of trace living cervical cancer cells. We achieved transient detection of the terahertz pulse time‐domain waveform of the living HeLa cells. The characteristic absorption peaks were identified by Lambert‐Beer law, respectively, at 0.49, 0.71, 1.04, 1.07, 1.26 and 1.37 THz. The absorbance is proportional to the cell concentration.
中文翻译：
瞬态太赫兹光谱技术检测活的宫颈癌细胞。
太赫兹波的光子能量与有机和生物大分子的旋转能级和振动能级处于同一数量级，因此在检测细胞和生物大分子方面具有独特的优势。然而，在生命环境中，细胞与环境相互作用的动态时标和生物大分子的结构构象变化在皮秒至毫秒之内，并且水对太赫兹波具有强吸收性，这已成为检测细胞和细胞的瓶颈问题。太赫兹技术的生物大分子。在本文中，我们基于光栅脉冲技术的倾斜波阵面开发了一套太赫兹单测量系统。该系统用于太赫兹痕量活宫颈癌细胞的太赫兹检测。我们实现了对活HeLa细胞太赫兹脉冲时域波形的瞬态检测。通过Lambert-Beer定律分别确定了特征吸收峰，分别为0.49、0.71、1.04、1.07、1.26和1.37 THz。吸光度与细胞浓度成正比。
更新日期：2020-08-30
中文翻译：
瞬态太赫兹光谱技术检测活的宫颈癌细胞。
太赫兹波的光子能量与有机和生物大分子的旋转能级和振动能级处于同一数量级，因此在检测细胞和生物大分子方面具有独特的优势。然而，在生命环境中，细胞与环境相互作用的动态时标和生物大分子的结构构象变化在皮秒至毫秒之内，并且水对太赫兹波具有强吸收性，这已成为检测细胞和细胞的瓶颈问题。太赫兹技术的生物大分子。在本文中，我们基于光栅脉冲技术的倾斜波阵面开发了一套太赫兹单测量系统。该系统用于太赫兹痕量活宫颈癌细胞的太赫兹检测。我们实现了对活HeLa细胞太赫兹脉冲时域波形的瞬态检测。通过Lambert-Beer定律分别确定了特征吸收峰，分别为0.49、0.71、1.04、1.07、1.26和1.37 THz。吸光度与细胞浓度成正比。
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