Can the mammalian organoid technology be applied to the insect gut?,Pest Management Science

当前位置： X-MOL 学术 › Pest Manag. Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Can the mammalian organoid technology be applied to the insect gut?
Pest Management Science ( IF 3.8 ) Pub Date : 2020-08-31 , DOI: 10.1002/ps.6067
Luc Swevers ₁ , Shane Denecke ₂ , Kathrin Vogelsang ₃ , Sven Geibel ₃ , John Vontas _{2,

4}

Affiliation

Mammalian intestinal organoids are multicellular structures that closely resemble the structure of the intestinal epithelium and can be generated in vitro from intestinal stem cells under appropriate culture conditions. This technology has transformed pharmaceutical research and drug development in human medicine. For the insect gut, no biotechnological platform equivalent to organoid cultures has been described yet. Comparison of the regulation of intestinal homeostasis and growth between insects and mammals has revealed significant similarities but also important differences. In contrast to mammals, the differentiation potential of available insect cell lines is limited and can not be exploited for in vitro permeability assays to measure the uptake of insecticides. The successful development of in vitro models could be a result of the emergence of molecular mechanisms of self‐organization and signaling in the intestine that are unique to mammals. It is nevertheless considered that the technology gap is a consequence of vast differences in knowledge, particularly with respect to culture conditions that maintain the differentation potential of insect midgut cells. From the viewpoint of pest control, advanced in vitro models of the insect midgut would be very desirable because of its key barrier function for orally ingested insecticides with hemolymphatic target and its role in insecticide resistance. © 2020 Society of Chemical Industry

中文翻译：

哺乳动物类器官技术可以应用于昆虫肠道吗？

哺乳动物肠道类器官是多细胞结构，与肠道上皮的结构非常相似，可以在适当的培养条件下从肠道干细胞体外产生。这项技术已经改变了人类医学领域的药物研究和药物开发。对于昆虫肠道，尚未描述与类器官培养物等效的生物技术平台。昆虫和哺乳动物之间肠道稳态和生长调节的比较显示出显着的相似点，但也有重要的区别。与哺乳动物相反，可利用的昆虫细胞系的分化潜能是有限的，不能用于体外。渗透性测定法来测量杀虫剂的吸收。体外模型的成功开发可能是哺乳动物独特的肠道自组织和信号传导分子机制出现的结果。然而，人们认为技术差距是知识差异巨大的结果，特别是在保持昆虫中肠细胞分化潜能的培养条件方面。从害虫控制的角度来看，昆虫中肠的先进体外模型将是非常理想的，因为它对具有血淋巴目标的口服摄取的杀虫剂具有关键的屏障功能，并且在杀虫剂抗性中具有作用。©2020化学工业协会

更新日期：2020-08-31

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11