The interaction between circRNAs and atherosclerosis has been extensively studied. However, more novel circRNAs need to be explored to help establish a perfect regulatory network. In the present research, hsa_circ_0000345 was demonstrated to regulate cellular development of oxygenized low‐density lipoprotein (ox‐LDL)‐treated aortic smooth muscle cells (ASMCs), which was closely related to the occurrence and progress of atherosclerosis. Ox‐LDL exposure remarkably decreased hsa_circ_0000345 expression in ASMCs. Transfection‐induced hsa_circ_0000345 overexpression activated cell viability (detected by an MTT assay) and restrained cellular apoptosis (analysed by flow cytometry) in the atherosclerosis cellular model. While down‐regulation of hsa_circ_0000345 reduced cell viability and promoted cell apoptosis. In addition, the data of the cell cycle distribution analysis and trans‐well assay indicated that cell cycle progression was arrested at the G1 phase while cell invasion was enhanced in ASMCs following treatment of ox‐LDL in the context of hsa_circ_0000345 OE plasmids. In addition, up‐regulation of hsa_circ_0000345 supported HIF‐1α at both the mRNA and protein level, and down‐regulation of hsa_circ_0000345 reduced HIF‐1α expression. Overall, the above findings revealed that hsa_circ_0000345 was a dramatic regulator of ASMCs proliferation, apoptosis and invasion in response to ox‐LDL treatment. Hsa_circ_0000345 was identified as a protector of cell viability during ox‐LDL induced cell development.

中文翻译：

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Hsa_circ_0000345调节ASMC对氧化的低密度脂蛋白的细胞发育。

circRNA与动脉粥样硬化之间的相互作用已得到广泛研究。但是，需要探索更多新颖的circRNA来帮助建立完善的调控网络。在本研究中，hsa_circ_0000345被证明可调节经氧合的低密度脂蛋白（ox-LDL）处理的主动脉平滑肌细胞（ASMC）的细胞发育，这与动脉粥样硬化的发生和发展密切相关。Ox-LDL暴露显着降低了ASMC中的hsa_circ_0000345表达。转染诱导的hsa_circ_0000345过表达激活了动脉粥样硬化细胞模型中的细胞活力（通过MTT分析检测）并抑制了细胞凋亡（通过流式细胞仪分析）。同时下调hsa_circ_0000345会降低细胞活力并促进细胞凋亡。此外，细胞周期分布分析和trans-well分析的数据表明，在hsa_circ_0000345 OE质粒处理ox-LDL后，ASMC中细胞周期进程被阻滞在G1期，而细胞侵袭则得以增强。此外，hsa_circ_0000345的上调在mRNA和蛋白质水平上均支持HIF-1α，而hsa_circ_0000345的下调则降低了HIF-1α的表达。总体而言，上述发现表明，hsa_circ_0000345是对ox-LDL处理的反应中ASMC增殖，凋亡和侵袭的重要调节剂。Hsa_circ_0000345被认为是ox-LDL诱导的细胞发育过程中细胞活力的保护者。