The clinical use of FDA‐approved bone morphogenetic proteins (BMPs) are impeded by high costs, super‐high dosage requirement, short half‐life, and other undesirable side effects. Therefore, designing a biomaterial that can promote new bone formation without using exogenous BMPs is highly desirable in clinical applications. In the present work, a new kind of nanofibrous scaffold composed of gelatin and 45S5 bioglass (GF/45S5 BG) was prepared through thermally induced phase separation method together with the particle leach technique (TIPS&P). In addition to the significantly higher mechanical strength, the composite scaffolds (GF/45S5 BG) significantly increased osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro compared with the neat scaffold (GF) without adding other biological agents, for example, BMPs or hormones. Most importantly, our in vivo studies also indicated that GF/45S5 BG scaffolds could directly promote ectopic bone regeneration in SD rats without exogenous BMP2. In summary, both in vitro and in vivo results indicated that the novel 45S5 bioglass functionalized GF nanofibrous scaffold is a promising alternative for bone tissue engineering.

中文翻译：

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用于骨再生的新型三维生物玻璃功能化明胶纳米纤维支架

FDA 批准的骨形态发生蛋白 (BMP) 的临床应用受到高成本、超高剂量要求、短半衰期和其他不良副作用的阻碍。因此，在临床应用中非常需要设计一种无需使用外源 BMP 即可促进新骨形成的生物材料。在目前的工作中，通过热诱导相分离法和颗粒浸出技术（TIPS&P）制备了一种由明胶和45S5生物玻璃组成的新型纳米纤维支架（GF/45S5 BG）。除了显着更高的机械强度外，与未添加其他生物制剂（例如 BMP）的纯支架 (GF) 相比，复合支架 (GF/45S5 BG) 在体外显着增加人间充质干细胞 (hMSC) 的成骨分化或荷尔蒙。最重要的是，我们的体内研究还表明，GF/45S5 BG 支架可以在没有外源性 BMP2 的情况下直接促进 SD 大鼠的异位骨再生。总之，体外和体内结果表明，新型 45S5 生物玻璃功能化 GF 纳米纤维支架是骨组织工程的一种有前途的替代品。