4-Thiouridine Enhanced Peroxidase-generated Biotinylation of RNA.,ChemBioChem

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4-Thiouridine Enhanced Peroxidase-generated Biotinylation of RNA.
ChemBioChem ( IF 2.6 ) Pub Date : 2020-08-30 , DOI: 10.1002/cbic.202000567
Jinguo Huang ₁ , Ruiqi Zhao ₁ , Shanshan Qin ₁ , Shixi Yang ₁ , Wei Li ₁ , Jing Mo ₁ , Fang Wang ₂ , Yuhao Du ₁ , Xiaocheng Weng ₁ , Xiang Zhou ₁

Affiliation

Subcellular, not substandard: A 4‐thiouridine‐enhanced RNA proximity biotinylation method that combines peroxidase‐mediated catalysis and s⁴U metabolic strategy has been developed to significantly enhance the efficiency of peroxidase‐generated RNA labeling. This method is easy to operate, benefitting RNA isolation and the study of the spatial transcriptome.