Here we have generated two induced pluripotent stem cell (iPSC) lines, hASC-iPSC-1A and hASC-iPSC-2A, by reprogramming human adipose tissue derived stem cells of 28 and 23 years old healthy donors. Reprogramming was achieved using nonintegrative Sendai viral vector system containing the reprogramming factors Klf4, Oct3/4, Sox2, c-Myc. Though the karyotypes of these cells were normal (46, XX) and (46, XY), their pluripotency potentials were confirmed by the expression of factors of pluripotency markers in vitro and teratoma formation in vivo. These iPSCs differentiated cells can serve as control for disease modeling and drug screening.

在这里，我们通过对28岁和23岁健康人的脂肪组织干细胞进行重新编程，生成了两种诱导性多能干细胞（iPSC）系hASC-iPSC-1A和hASC-iPSC-2A。使用包含重编程因子Klf4，Oct3 / 4，Sox2，c-Myc的非整合型仙台病毒载体系统实现了重编程。尽管这些细胞的核型是正常的（46，XX）和（46，XY），但其多能性潜力已通过体外多能性标记因子的表达和体内畸胎瘤的形成得到证实。这些iPSC分化的细胞可以用作疾病建模和药物筛选的对照。