Levan (LVN) is a fructan homopolysaccharide biopolymer which has the unique properties for development of a biosensor platform. Herein, LVN modified disposable electrochemical biosensor was developed for detection of DNA, daunorubicin (DNR) and the biomolecular interaction of DNR with DNA. For this purpose, pencil graphite electrodes (PGE) were used as biosensor platform. The microscopic and electrochemical characterization of LVN-PGEs were done in contrast to the control group and the results were showed that the LVN was successfully modified onto the PGE surface. The optimization of experimental conditions such as LVN concentration and DNA concentration was performed. The detection limit was estimated as 2.74 μg/mL using the equation I = 0.05 x C_fsDNA+ 3.54 with the R² = 0.99 for DNA using LVN-PGEs. DNR-DNA interaction was investigated under the optimum conditions. Robust, sensitive, biocompatible and disposable biosensor surface was achieved by modification of PGE surface using LVN biopolymer for electrochemical monitoring of DNA, anticancer agent, DNR as well as DNA-DNR interaction. The single-use electrochemical DNA biosensor modified with LVN is a model for further studies on the development of novel biopolymers based biosensors.

Levan（LVN）是一种果聚糖均多糖生物聚合物，具有开发生物传感器平台的独特性能。本文中，开发了LVN修饰的一次性电化学生物传感器，用于检测DNA，柔红霉素（DNR）和DNR与DNA的生物分子相互作用。为此，笔形石墨电极（PGE）被用作生物传感器平台。与对照组相比，对LVN-PGE进行了显微和电化学表征，结果表明LVN已成功修饰到PGE表面。进行实验条件的优化，例如LVN浓度和DNA浓度。使用方程式I = 0.05 x C _fsDNA + 3.54（R ²）估计检测限为_2.74μg / mL 对于使用LVN-PGE的DNA = 0.99。在最佳条件下研究了DNR-DNA的相互作用。通过使用LVN生物聚合物对PGE表面进行修饰来实现DNA的电化学监测，抗癌剂，DNR以及DNA-DNR相互作用，从而获得了坚固，灵敏，具有生物相容性的一次性生物传感器表面。LVN修饰的一次性电化学DNA生物传感器是用于进一步研究基于新型生物聚合物的生物传感器的模型。