Biotic and abiotic elicitors have been reported to promote growth or multiplication as well as alter bioactive compositions of different plant species. Lavandula officinalis is a perennial plant, which produces fragrant flowers that are utilized to prepare the lavender oil. Lavender oil is rich in beta-linalool, cineol, camphor and many other bioactive compounds, which together contributes to its pain relieving, antimicrobial and cicatrizing activities. Micropropagation is a good alternative to propagate lavender as it reduces the time required to achieve the production of essential oil and eliminates the exposures of the lavender plants to environmental pollutants, thus ensuring a better quality of the oil produced. In this study, we have investigated the effects of 3 biotic elicitors - Moringa oleifera leaf extract (MLE), Aloe vera leaf extract (ALE), Spirulina platensis aqueous extract (SAE) and an abiotic elicitor - Sodium chloride (NaCl) on the growth, multiplication and phytochemical compositions of Lavandula officinalis in-vitro plantlets. All tested biotic elicitors (MLE, ALE, SAE) negatively affected the plantlets fresh weight and shoots height. However, these elicitors were found to enhance the number of leaves per explant. On the other hand, the effect of abiotic elicitor (NaCl) were opposite to that of the biotic elicitors. In contrast to the negative growth effects, SAE and high ALE concentration (40 ml/L) were found to enhance number of shoots per explant. Interestingly, we found that all tested biotic and abiotic elicitors were able to increase the beta-linalool contents in the L. officinalis in-vitro plantlets. We observed that SAE is the best elicitor among the 4 tested elicitors because both 0.5 and 1.0 g/L SAE were able to increase linalool production and enhanced shoots proliferation.

据报道，生物和非生物引发剂可促进生长或繁殖以及改变不同植物物种的生物活性组成。薰衣草是多年生植物，其产生芬芳的花朵，用于制备薰衣草油。薰衣草油含有丰富的β-芳樟醇，桉树脑，樟脑和许多其他生物活性化合物，它们共同有助于其止痛，抗微生物和疤痕愈合活性。微繁殖是繁殖薰衣草的一种很好的选择，因为它减少了实现精油生产所需的时间，并且消除了薰衣草植物对环境污染物的暴露，从而确保了所生产油的更好质量。在这项研究中，我们研究了3种生物激发子的作用-辣木叶提取物（MLE），芦荟叶提取物（ALE），螺旋藻水提取物（SAE）和非生物激发子-氯化钠（NaCl）对紫叶薰衣草的生长，繁殖和植物化学成分的影响体外苗。所有测试的生物激发子（MLE，ALE，SAE）均会对小苗的鲜重和苗高产生负面影响。然而，发现这些激发子提高了每个外植体的叶片数量。另一方面，非生物激发子（NaCl）的作用与生物激发子相反。与负面的生长效应相反，发现SAE和高ALE浓度（40 ml / L）可以增加每个外植体的芽数。有趣的是，我们发现，所有被测试的生物和非生物诱导能够提高在公测芳樟醇含量L.巴戟体外植株。我们观察到SAE是4种受试激发子中最好的激发子，因为0.5和1.0 g / L SAE都能增加芳樟醇的产量并增强枝条的增殖。