Ammonia, as one of the major environmental pollutants in aquaculture, can lead to negative impacts on health and production of farmed animals. However, the underlying molecular mechanism of ammonia in crayfish remains unclear. In this study, the transcriptomics of hemolymph, gill and hepatopancreas of subjected to ammonia stress were analyzed through comparison with a control group. After assembly and annotation, a total of 87,674 unigenes were obtained with an average length of 1283 bp. A total of 5062 differentially expressed genes (DEGs) were detected, including 2140 significantly upregulated DEGs and 2922 significantly downregulated DEGs. The expression of these DEGs was verified by 10 randomly selected genes using the RT-qPCR technique. Many genes related to the purine metabolism pathway, such as adenylate kinase (ADK), adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP), and allantoinase (ALN), were downregulated, whereas the expression of adenosine monophosphate deaminase (AMPD) was upregulated. Genes related to oxidant-antioxidant equilibrium, such as Cu/Zn superoxide dismutase (SOD), peroxiredoxin-1(PRDX1), and catalase (CAT), were significantly downregulated. cytochrome C (Cyt-C) and cathepsins, which play important roles in apoptosis, were significantly upregulated. Collectively, ammonia stress significantly changed purine metabolism pathway, destabilized the oxidant-antioxidant equilibrium, and caused apoptosis in . To maintain normal life activities, reduced purine metabolism and increased deamination of AMP to cope with the influence of ammonia stress. These results lay a foundation for further study on the mechanisms of the response of to ammonia stress and provide theoretical guidance for the sustainable aquaculture of this species.

中文翻译：

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RNA-seq 分析揭示了氨对克氏原螯虾（Procambarus clarkii）新陈代谢、氧化-抗氧化平衡和细胞凋亡的影响


氨作为水产养殖中的主要环境污染物之一，会对养殖动物的健康和生产产生负面影响。然而，小龙虾中氨的潜在分子机制仍不清楚。在本研究中，通过与对照组进行比较，对氨胁迫下的血淋巴、鳃和肝胰腺的转录组学进行了分析。经过组装和注释，共获得87,674个unigenes，平均长度为1283 bp。共检测到5062个差异表达基因（DEG），其中显着上调的DEG 2140个，显着下调的DEG 2922个。使用 RT-qPCR 技术通过 10 个随机选择的基因验证这些 DEG 的表达。腺苷酸激酶（ADK）、腺苷脱氨酶（ADA）、嘌呤核苷磷酸化酶（PNP）、尿囊素酶（ALN）等嘌呤代谢途径相关基因下调，而单磷酸腺苷脱氨酶（AMPD）表达下调。上调。与氧化剂-抗氧化剂平衡相关的基因，如铜/锌超氧化物歧化酶（SOD）、过氧化还原蛋白-1（PRDX1）和过氧化氢酶（CAT）显着下调。在细胞凋亡中起重要作用的细胞色素C (Cyt-C)和组织蛋白酶显着上调。总的来说，氨应激显着改变了嘌呤代谢途径，破坏了氧化剂-抗氧化剂平衡，并导致细胞凋亡。维持正常的生命活动，减少嘌呤代谢，增加AMP脱氨以应对氨应激的影响。这些结果为进一步研究该物种对氨胁迫的响应机制奠定了基础，并为该物种的可持续养殖提供理论指导。