The sugarcane (Saccharum X officinarum) is one of the most important crops used to produce sugar and raw material for biofuel in the world. One of the main causes for sucrose content and yield losses is the attack by insect. In this investigation, cry1Ac gene was introduced into sugarcane variety GT54-9(C9) using the Agrobacterium tumefaciens transformation method for transgenic sugarcane production presenting insect-resistance. The A. tumefaciens strain GV1303 including pARTcry1Ac vector was used for the production of transformed sugarcane. The Bacillus thuringiensis cry gene were successfully used to produce transgenic plants used for the improvement of both agronomic efficiency and product quality by acquiring insect resistance. PCR and Southern hybridization techniques were used to confirm the cry1Ac gene incorporation into sugarcane genome. Transformation percentage was 22.2% using PCR analysis with specific primers for cry1Ac and npt-II (Neomycin phosphotransferase) genes. The expression of cry1Ac gene was determined using reverse transcriptase polymerase chain reaction (RT-PCR), QuickStix test, and insect bioassays. Bioassays for transformed sugarcane plants showed high level of toxicity to Sesamia cretica giving 100% mortality of the larvae. Sugarcane insect resistance was improved significantly by using cry1Ac gene transformation.

甘蔗（Saccharum X officinarum）是世界上用于生产糖和生物燃料原料的最重要的作物之一。蔗糖含量和产量损失的主要原因之一是昆虫的侵袭。在这项研究中，使用根癌农杆菌转化方法将cry1Ac基因导入甘蔗品种GT54-9(C9)，用于生产具有抗虫性的转基因甘蔗。的根癌农杆菌菌株GV1303包括部分cry1Ac基因的载体用于生产转化的甘蔗。将苏云金芽孢杆菌哭基因被成功地用于生产转基因植物，用于通过获得昆虫抗性来提高农艺效率和产品质量。PCR和Southern杂交技术用于确认cry1Ac基因掺入甘蔗基因组。使用针对cry1Ac和npt-II（新霉素磷酸转移酶）基因的特异性引物进行 PCR 分析，转化率为 22.2% 。使用逆转录酶聚合酶链反应 (RT-PCR)、QuickStix 测试和昆虫生物测定法测定了cry1Ac基因的表达。转化甘蔗植物的生物测定显示对芝麻具有高度毒性使幼虫的死亡率为 100%。通过使用cry1Ac基因转化，甘蔗抗虫性显着提高。