The ability to trap precise quantities of cells or particles into confined areas has numerous applications for biological purposes. In particular, single cell trapping has received considerable attention because it permits the study of heterogeneity in a population, while trapping larger groups of cells have been used to form aggregates. Among several methods, the use of microwell offers a simple platform to capture cells or particles using hydrodynamic forces. This review examines the use of microwells in both static and microfluidic environments, and the application of microfluidic geometric arrays for trapping. This paper discusses the design and fabrication methods of microwells and compares the trapping and release techniques used in both static and microfluidics‐integrated microwells. Finally, we will summarize novel microfluidic geometric arrays used to capture cells or particles through hydrodynamic trapping.

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微孔和微流控几何阵列制造技术及其在细胞研究中的潜在应用综述

将精确数量的细胞或颗粒捕获到狭窄区域的能力在生物学上有许多应用。特别地，单细胞捕获已引起相当大的关注，因为它允许研究群体中的异质性，而捕获更大的细胞群已被用来形成聚集体。在几种方法中，微孔的使用提供了一个简单的平台，可以利用流体动力来捕获细胞或颗粒。这篇综述探讨了在静态和微流体环境中微孔的使用，以及微流体几何阵列在捕集中的应用。本文讨论了微孔的设计和制造方法，并比较了在静态和微流体集成微孔中使用的捕获和释放技术。最后，