Type 1 diabetes is a metabolic disorder caused by the loss or dysfunction of β‐cells in the pancreas. Organ shortage is a critical concern of diabetic patients in need of beta islet transplantation. Tissue engineered islets are promising alternatives to traditional organ transplantation. Recent progress in stem cell biology and gene cloning techniques has raised hopes for the generation of insulin producing cells (IPCs) without the need of immunosuppression. The purpose of this study was to produce IPCs using human adipose‐derived stem cells (hADSCs) and human endometrial‐derived stem cells (hEnSCs) and also to compare the level of insulin secretion by these cells in 2D and 3D culture systems on fibrin scaffolding. Stem cells differentiation was carried out through transduction with an insulin over expression lentiviral vector. Real‐time PCR and immunocytochemistry confirmed the successful transduction of both cell types. Both cell types showed comparable insulin secretion by ELISA.3D culture resulted in higher amounts of insulin secretion of the two cell types versus 2D as control. This study showed that insulin gene delivery to the stem cells could be an efficient method for producing IPCs and fibrin encapsulation enhances the functionality of these cells.

中文翻译：

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纤维蛋白支架上 2D 和 3D 培养物中转导的脂肪源性干细胞和子宫内膜源性干细胞分泌胰岛素的比较

1 型糖尿病是一种由胰腺中 β 细胞丢失或功能障碍引起的代谢紊乱。器官短缺是需要β胰岛移植的糖尿病患者的一个关键问题。组织工程胰岛是传统器官移植的有前途的替代品。干细胞生物学和基因克隆技术的最新进展为无需免疫抑制的胰岛素生产细胞 (IPC) 的生成带来了希望。本研究的目的是使用人脂肪源性干细胞 (hADSCs) 和人子宫内膜源性干细胞 (hEnSCs) 生产 IPC，并比较这些细胞在纤维蛋白支架上的 2D 和 3D 培养系统中的胰岛素分泌水平. 通过用胰岛素过表达慢病毒载体转导进行干细胞分化。实时 PCR 和免疫细胞化学证实了两种细胞类型的成功转导。通过ELISA，两种细胞类型显示出相当的胰岛素分泌。与作为对照的2D相比，3D培养导致两种细胞类型的胰岛素分泌量更高。该研究表明，将胰岛素基因递送至干细胞可能是产生 IPC 的有效方法，而纤维蛋白封装可增强这些细胞的功能。