Inspired by the biomimetic modification strategy of dopamine self-polymerization technique, molecularly imprinted nanocomposite membranes (MINCMs) with thermo-responsive rebinding and separation performance were synthesized and evaluated. Herein, the Au/SiO₂-based multilevel structure had been successfully obtained onto the polydopamine (pDA) modified membrane surfaces. Afterward, the poly(N-isopropylacrylamide)-based biomolecule-imprinted sites were adequately constructed by developing a photoinitiated atom transfer radical polymerization (pATRP) imprinting strategy using the high-biocompatible ovalbumin (Ova, pI 4.6) as template molecule. Therefore, thermo-responsive ‘specific recognition sites’ toward Ova were then achieved on the as-prepared MINCMs after the well-designed imprinting process. When the external temperature was set at 37 °C, excellent ovalbumin rebinding capacity (33.26 mg/g), selectivity factor (3.06) and structural stability were obtained. Importantly, as to the controllable biocompatibility research of this work, the bare glass and Ova-bound-MINCMs (the MINCMs were bound with Ova) showed basically the same cell adhesion behaviors and viability, indicating the excellent biocompatibility of the Ova-bound-MINCMs. Additionally, efficient and rapid regulation of cell adhesion/detachment on ovalbumin-bound MINCMs could be still obtained even after 10 cycles of temperature-switch process, which indicated that the as-prepared MINCMs had strong ability to work under high intensity and long continuous operation.

受多巴胺自聚合技术仿生修饰策略的启发，合成并评估了具有热响应性重新结合和分离性能的分子印迹纳米复合膜（MINCM）。在此，已经成功地在聚多巴胺（pDA）修饰的膜表面上获得了基于Au / SiO ₂的多层结构。之后，poly（N通过使用高生物相容性卵清蛋白（Ova，pI 4.6）作为模板分子开发光引发的原子转移自由基聚合（pATRP）印迹策略来适当构建基于-异丙基丙烯酰胺）的生物分子印迹位点。因此，经过精心设计的压印过程后，在准备好的MINCM上实现了对Ova的热响应性“特异性识别位点”。当外部温度设定为37℃时，获得了优异的卵清蛋白再结合能力（33.26mg / g），选择性因子（3.06）和结构稳定性。重要的是，对于这项工作的可控生物相容性研究，裸玻璃和与Ova结合的MINCM（MINCM与Ova结合）显示出基本相同的细胞粘附行为和生存能力，这表明与Ova结合的MINCM具有出色的生物相容性。 。