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Immunosensor incorporating half-antibody fragment for electrochemical monitoring of amyloid-β fibrils in artificial blood plasma.
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2020-08-29 , DOI: 10.1016/j.bioelechem.2020.107643
Gopal Palla 1 , Kamila Malecka 1 , Wim Dehaen 2 , Jerzy Radecki 1 , Hanna Radecka 1
Affiliation  

In this report, an electrochemical immunosensor for the selective and sensitive monitoring of Aβ1-42 fibrils is presented. The sensing platform was prepared by the formation of a 4,4′-thiobisbenzenethiol (TBBT) self-assembled monolayer on a clean gold surface followed by the covalent entrapment of gold nanoparticles (AuNPs). The half-antibody fragments of the Anti-Amyloid Fibrils antibody were immobilized on AuNPs via S-Au covalent bonds. Each step of immunosensor fabrication was characterized with cyclic voltammetry and electrochemical impedance spectroscopy. The biosensor was successfully used for the sensing of Aβ1-42 fibrils in both phosphate saline buffer (PBS) and artificial blood plasma (ABP). The immunosensor sensitivity estimated based on calibration slopes was better in the presence of APP in the comparison to PBS. The LOD values obtained for both measuring media were of 0.6 pM level. The moderate response towards Aβ1-42 oligomers demonstrated the immunosensor selectivity.



中文翻译:

结合了半抗体片段的免疫传感器,用于在人工血浆中电化学监测淀粉样β蛋白原纤维。

在该报告中,提出了一种用于选择性和灵敏监测Aβ1-42原纤维的电化学免疫传感器。通过在干净的金表面上形成4,4'-硫代双苯硫醇(TBBT)自组装单层,然后共价捕获金纳米颗粒(AuNPs),来制备传感平台。抗淀粉样蛋白原纤维抗体的半抗体片段通过S-Au共价键固定在AuNPs上。免疫传感器制造的每个步骤都通过循环伏安法和电化学阻抗谱进行了表征。该生物传感器成功用于Aβ1-42的传感磷酸盐盐水缓冲液(PBS)和人工血浆(ABP)中的原纤维。在存在APP的情况下,与PBS相比,基于校准斜率估算的免疫传感器灵敏度更好。两种测量介质的LOD值均为0.6 pM。对Aβ1-42寡聚物的中等反应表明了免疫传感器的选择性。

更新日期:2020-09-05
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