Abstract

Hami melon (Cucumis melo L. var. inodorus Jacq) is an important horticultural crop but susceptible to chilling injury during low temperature storage. To characterize the gene expression profiles of Gold Queen Hami melon under the cold stress and discover the key cold stress-induced genes, five cDNA libraries, C0 (control), C12 (control for 12 d), C24 (control for 24 d), L12 (cold treatment at 0.5°C for 12 d) and L24 (cold treatment at 0.5°C for 24 d), were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. A total of 6479 and 7914 differentially expressed genes (DEGs) in L12 and L24 compared with C0 were respectively detected. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses identified the relevant biological functions and metabolic pathways of these differentially expressed genes. In addition, a diverse range of cold response candidate genes were mainly obtained from starch and sucrose metabolism, pentose phosphate pathway and glycolysis/gluconeogenesis, such as genes encoding sucrose-phosphate synthase, α-amylase, β-amylase, sucrose synthase and glucose-6-phosphate dehydrogenase. Various transcription factors with divergent expression patterns were identified. Finally, quantitative real time polymerase chain reaction (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. This study provides valuable information for further studies on cold response mechanisms and genetic improvement of cold tolerance in Gold Queen Hami melons.

中文翻译：

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低温胁迫下哈密瓜女王的转录组谱分析

摘要

哈密​​瓜（Cucumis melo L. var。inodorus雅克（Jacq）是重要的园艺作物，但在低温储存期间易受冻害。为了表征冷胁迫下金皇后哈密瓜的基因表达谱并发现关键的冷胁迫诱导基因，五个cDNA文库，C0（对照），C12（对照12 d），C24（对照24 d），构建L12（在0.5°C下冷处理12 d）和L24（在0.5°C下冷处理24 d）以进行RNA测序（RNA-Seq）和基因表达谱分析。与C0相比，在L12和L24中总共检测到6479和7914个差异表达基因（DEG）。基因本体论（GO）和《京都基因与基因组百科全书》（KEGG）富集分析确定了这些差异表达基因的相关生物学功能和代谢途径。此外，各种各样的冷应答候选基因主要来自淀粉和蔗糖代谢，磷酸戊糖途径和糖酵解/糖异生，例如编码蔗糖磷酸合酶，α-淀粉酶，β-淀粉酶，蔗糖合酶和葡萄糖-6-磷酸的基因。脱氢酶。鉴定了具有不同表达模式的各种转录因子。最后，进行定量实时聚合酶链反应（qRT-PCR）以验证RNA-Seq和基因差异表达结果。这项研究为进一步研究金皇后哈密瓜的冷响应机制和耐冷性的遗传改良提供了有价值的信息。例如编码蔗糖磷酸合酶，α-淀粉酶，β-淀粉酶，蔗糖合酶和葡萄糖-6-磷酸脱氢酶的基因。鉴定了具有不同表达模式的各种转录因子。最后，进行定量实时聚合酶链反应（qRT-PCR）以验证RNA-Seq和基因差异表达结果。这项研究为进一步研究金皇后哈密瓜的冷响应机制和耐冷性的遗传改良提供了有价值的信息。例如编码蔗糖磷酸合酶，α-淀粉酶，β-淀粉酶，蔗糖合酶和葡萄糖-6-磷酸脱氢酶的基因。鉴定了具有不同表达模式的各种转录因子。最后，进行定量实时聚合酶链反应（qRT-PCR）以验证RNA-Seq和基因差异表达结果。这项研究为进一步研究金皇后哈密瓜的冷响应机制和耐冷性的遗传改良提供了有价值的信息。