Abstract

In this study, effects of soy isoflavones (SIF) on the growth and development, physiology, content of medicinal ingredients and expression levels of enzyme-encoding genes in Achyranthes bidentata Blume under UV-B radiation were systematically investigated. The results showed that UV-B radiation adversely affected the growth and development of A. bidentata seedlings, causing ROS accumulation and the anti-oxidation system to be damaged. However, 1 mg/L exogenous SIF treatment conferred enhanced tolerance under UV-B radiation stress in A. bidentata seedlings by decreasing the concentration of MDA, H₂O_2, and modulating SOD and POD activities. Furthermore, 1 mg/L SIF reduced the content of oleanolic acid and ecdysterone in leaves and increased the content of oleanolic acid in roots, but had no significant effect on the content of ecdysterone in roots under UV-B radiation stress. SIF had a different degree of influence on the expression levels of antioxidant enzyme genes as well as medicinal ingredients enzyme-encoding genes in A. bidentata. The results showed that 1 mg/L exogenous SIF treatment had a positive effect on the growth, development and physiology in A. bidentata seedlings under UV-B radiation stress. To our knowledge, for the first time we are reporting that the use of 1 mg/L exogenous SIF enhances A. bidentata seedlings tolerance to UV-B radiation stress.

中文翻译：

---

UV-B辐射胁迫下外源大豆异黄酮对牛膝幼苗生理参数的影响

摘要

在这项研究中，系统地研究了大豆异黄酮（SIF）在UV-B辐射下对牛膝的生长，生理，药用成分含量和酶编码基因表达水平的影响。结果表明，UV-B辐射对A.bidentata幼苗的生长和发育有不利影响，导致ROS积累和抗氧化系统受到破坏。然而，通过降低MDA，H ₂ O ₂的浓度，采用1 mg / L外源SIF处理后，A.bidentata幼苗在UV-B辐射胁迫下耐受性增强_。并调节SOD和POD活动。此外，1 mg / L SIF降低了叶片中齐墩果酸和蜕皮甾酮的含量，增加了根中齐墩果酸的含量，但对UV-B辐射胁迫下根中的十八烷酮含量没有显着影响。SIF有不同程度的对抗氧化酶基因的表达水平的影响，以及编码酶作为药效成分基因牛膝。结果表明，在UV-B辐射胁迫下，外源SIF处理1 mg / L对A.bidentata幼苗的生长，发育和生理有积极影响。据我们所知，我们首次报告使用1 mg / L外源性SIF可以增强A. bidentata 幼苗对UV-B辐射胁迫的耐受性。