The objective of this study was to evaluate the influence of tetracyclines associated with swine wastewater on the enzymatic activity of soil over time, in relation to the biogeochemical cycle of the nutrients during two seasons of the year. The experiment was carried out in soil-containing pots that received two successive applications of swine wastewater treatments, in the summer and autumn seasons, corresponding to two experimental periods. The swine wastewater was applied in doses of 0.2 and 0.3 L, with or without tetracycline, chlortetracycline, and doxycycline. Soil samples were collected at 2, 4, 8, 15, 30, 45, and 75 days after treatment application to determine the activities of the enzymes dehydrogenase, β-glucosidase, acid phosphatase, and urease. During both experimental periods, there was an increase in the activities of all enzymes in the presence of swine wastewater. However, these activities decreased in the presence of the applied antibiotics. The observation of enzyme activity over time facilitated construction of longitudinal predictive models, which described the activity of enzymes over time. Our findings indicate that swine wastewater treatments led to a reduction in the bioavailability of nutrients, thereby reducing soil quality and fertility.

中文翻译：

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四环素对酶活性和土壤养分有效性的影响

本研究的目的是评估与猪废水相关的四环素随着时间的推移对土壤酶活性的影响，与一年中两个季节养分的生物地球化学循环有关。该实验在包含土壤的盆中进行，该盆在夏季和秋季接受了两次连续应用猪废水处理，对应于两个实验时期。猪废水以 0.2 和 0.3 L 的剂量施用，有或没有四环素、金霉素和强力霉素。在施用处理后 2、4、8、15、30、45 和 75 天收集土壤样品，以确定酶脱氢酶、β-葡萄糖苷酶、酸性磷酸酶和脲酶的活性。在两个实验期间，在存在猪废水的情况下，所有酶的活性都有所增加。然而，这些活性在应用抗生素的情况下降低。随着时间的推移对酶活性的观察促进了纵向预测模型的构建，该模型描述了酶的活性随时间的变化。我们的研究结果表明，猪废水处理导致养分生物利用度降低，从而降低土壤质量和肥力。