Here, we aim at developing a novel biomatrix from decellularized bovine spinal meninges for tissue engineering and regenerative medicine applications. Within this concept, the bovine spinal meninges were decellularized using 1% Triton X-100 for 48 h, and residual nuclear content was determined with double-strand DNA content analysis and agarose gel electrophoresis. The major matrix components such as sulfated GAGs and collagen before and after the decellularization process were analyzed with DMMB, hydroxyproline assay and SDS-PAGE. Subsequently, the native bovine spinal meninges (nBSM) and decellularized BSM (dBSM) were physiochemically characterized via ATR-FTIR spectroscopy, TGA, DMA and tensile strength test. The dsDNA content in the nBSM was 153.39 ± 53.93 ng/mg dry weight, versus in the dBSM was 39.47 ± 4.93 ng/mg (n = 3) dry weight and DNA fragments of more than 200 bp in length were not detected in the dBSM by agarose gel electrophoresis. The sulfated GAGs contents for nBSM and dBSM were observed to be 10.87 ± 1.2 and 11.42 ± 2.01 μg/mg dry weight, respectively. The maximum strength of dBSM in dry and wet conditions was found to be 19.67 ± 0.21 MPa and 13.97 ± 0.17 MPa, while nBSM (dry) was found to be 26.26 ± 0.28 MPa. MTT, SEM, and histology results exhibited that the cells attached to the surface of dBSM, and proliferated on the dBSM. In conclusion, the in vitro preliminary study has demonstrated that the dBSM might be a proper and new bioscaffold for tissue engineering and regenerative medicine applications.

在这里，我们的目标是开发一种用于组织工程和再生医学应用的脱细胞牛脊髓膜的新型生物基质。在这个概念内，牛脊髓膜使用 1% Triton X-100 脱细胞 48 小时，残留核含量通过双链 DNA 含量分析和琼脂糖凝胶电泳确定。脱细胞过程前后的主要基质成分如硫酸化 GAG 和胶原蛋白用 DMMB、羟脯氨酸测定和 SDS-PAGE 分析。随后，通过 ATR-FTIR 光谱、TGA、DMA 和拉伸强度测试对天然牛脊髓膜 (nBSM) 和脱细胞 BSM (dBSM) 进行了理化表征。nBSM 中的 dsDNA 含量为 153.39 ± 53.93 ng / mg 干重，而 dBSM 中的含量为 39.47 ± 4。通过琼脂糖凝胶电泳在 dBSM 中未检测到 93 ng / mg (n = 3) 干重和长度超过 200 bp 的 DNA 片段。观察到 nBSM 和 dBSM 的硫酸化 GAG 含量分别为 10.87 ± 1.2 和 11.42 ± 2.01 μg/mg 干重。发现 dBSM 在干湿条件下的最大强度为 19.67 ± 0.21 MPa 和 13.97 ± 0.17 MPa，而 nBSM（干）为 26.26 ± 0.28 MPa。MTT、SEM和组织学结果表明细胞附着在dBSM表面，并在dBSM上增殖。总之，体外初步研究表明，dBSM 可能是一种适用于组织工程和再生医学应用的新型生物支架。观察到 nBSM 和 dBSM 的硫酸化 GAG 含量分别为 10.87 ± 1.2 和 11.42 ± 2.01 μg/mg 干重。发现 dBSM 在干湿条件下的最大强度为 19.67 ± 0.21 MPa 和 13.97 ± 0.17 MPa，而 nBSM（干）为 26.26 ± 0.28 MPa。MTT、SEM和组织学结果表明细胞附着在dBSM表面，并在dBSM上增殖。总之，体外初步研究表明，dBSM 可能是一种适用于组织工程和再生医学应用的新型生物支架。观察到 nBSM 和 dBSM 的硫酸化 GAG 含量分别为 10.87 ± 1.2 和 11.42 ± 2.01 μg/mg 干重。发现 dBSM 在干湿条件下的最大强度为 19.67 ± 0.21 MPa 和 13.97 ± 0.17 MPa，而 nBSM（干）为 26.26 ± 0.28 MPa。MTT、SEM和组织学结果表明细胞附着在dBSM表面，并在dBSM上增殖。总之，体外初步研究表明，dBSM 可能是一种适用于组织工程和再生医学应用的新型生物支架。组织学结果表明，细胞附着在dBSM表面，并在dBSM上增殖。总之，体外初步研究表明，dBSM 可能是一种适用于组织工程和再生医学应用的新型生物支架。组织学结果表明，细胞附着在dBSM表面，并在dBSM上增殖。总之，体外初步研究表明，dBSM 可能是一种适用于组织工程和再生医学应用的新型生物支架。