To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction.

中文翻译：

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二黄汤口服后吸收成分的归属及HPLC-DAD-ESI-MS鉴定

为实现半仿生酶提法制备的二黄汤口服后大鼠血清中吸收成分的归属和鉴定，采用HPLC-DAD-ESI-MS方法建立血清样品指纹图谱。口服二黄汤后，在二黄汤中检测到32个峰，在大鼠血清中检测到24个峰。在大鼠血清中检测到的24个峰中，通过与参考化合物的保留时间和质谱数据比较，或通过质谱分析并检索参考文献，鉴定出25个化合物。在体内鉴定出的25种化合物中，24种为体外吸收的化合物的原始形态，1种为甘草的代谢产物。通过质谱或紫外吸收特性分析，推断其他未鉴定的体内化合物为血清中的内源性代谢物或该化合物在体内存在的原始形态和代谢物。结果表明，HPLC-DAD-ESI-MS适用于二黄汤口服后血清中生物活性成分的鉴定，该研究结果将有利于二黄汤药效物质基础的进一步研究和开发。