LI85008F is a proprietary combination of leaf extracts of Moringa oleifera, Murraya koeingii, and extract of Curcuma longa rhizome. This herbal extract combination is an effective weight loss supplement for overweight and obese subjects. The present study aimed to investigate the thermogenic potential of the LI85008F in high-fat diet (HFD)-induced obese Sprague Dawley rats. Seven rats received a regular diet (RD), and twenty-one rats received a high-fat diet (HFD) for 56 days. On day 28, the HFD-fed rats were randomized into three groups (n = 7). Starting from day 29 through day 56, one HFD-fed group received daily oral gavage of 0.5% Carboxymethylcellulose Sodium (CMC) alone (HFD), and the remaining two groups received 100 and 250 mg/kg LI85008F (LI85008F-100 and LI85008F-250, respectively). Body weight, fat mass, fat cell size, liver weight, liver triglyceride were measured. The energy metabolism parameters were measured using indirect calorimetry. In serum, the metabolic and endocrine markers were analyzed. The adipogenic and thermoregulatory proteins expression in the white adipose tissue (WAT) were analyzed using an immunoblot assay. Supplementation with both doses of LI85008F significantly increased resting energy expenditure (REE) in the obese rats. The LI85008F-250 rats showed significant up-regulation of uncoupling protein-1 (UCP-1) expression, as compared with the HFD rats. LI85008F significantly reduced body weight gain, fat mass, fat cell size, liver weight, and hepatic triglycerides. Serum triglyceride, total cholesterol, glucose, leptin, and fat cell markers were significantly reduced in LI85008F-supplemented rats compared to the HFD rats. The present data suggest that LI85008F reduces body fat mass and controls body weight gain via increasing energy metabolism in combination with reduced lipogenesis in diet-fed obese rats.

中文翻译：

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辣木，Murraya koeingii叶子和姜黄根茎的联合提取物增加了高脂饮食喂养大鼠的能量消耗并控制了肥胖。

LI85008F是辣木叶提取物，香兰木叶提取物和姜黄根茎提取物的专有组合。这种草药提取物组合是超重和肥胖受试者的有效减肥补品。本研究旨在调查LI85008F在高脂饮食（HFD）诱导的肥胖Sprague Dawley大鼠中的产热潜力。7只大鼠接受常规饮食（RD），21只大鼠接受高脂饮食（HFD），持续56天。在第28天，由HFD喂养的大鼠被随机分为三组（n = 7）。从第29天到第56天，一个由HFD喂养的组每天接受单独0.5％羧甲基纤维素钠（CMD）的口服灌胃，其余两组分别接受100和250 mg / kg LI85008F（LI85008F-100和LI85008F- 250）。体重，脂肪量，脂肪细胞大小，肝脏重量，测量肝甘油三酸酯。使用间接量热法测量能量代谢参数。在血清中，分析了代谢和内分泌标志物。使用免疫印迹分析法分析白色脂肪组织（WAT）中的成脂蛋白和温度调节蛋白表达。两种剂量的LI85008F的补充均显着增加了肥胖大鼠的静息能量消耗（REE）。与HFD大鼠相比，LI85008F-250大鼠显示出未偶联蛋白1（UCP-1）表达的显着上调。LI85008F显着降低了体重增加，脂肪量，脂肪细胞大小，肝脏重量和肝甘油三酸酯。与HFD大鼠相比，补充LI85008F的大鼠的血清甘油三酸酯，总胆固醇，葡萄糖，瘦素和脂肪细胞标志物显着降低。