当前位置：
X-MOL 学术
›
Indian J. Med. Microbiol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Diagnostic utility of serology and polymerase chain reaction for detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in paediatric community-acquired lower respiratory tract infections.
Indian Journal of Medical Microbiology ( IF 1.4 ) Pub Date : 2020-04-01 , DOI: 10.4103/ijmm.ijmm_20_145 Sanchit Kumar 1 , Bineeta Kashyap 1 , Surinder Kumar 1 , Seema Kapoor 2
Purpose: Mycoplasma pneumoniae (M. pneumoniae) and Chlamydophila pneumoniae (C. pneumoniae) play a significant role in children of all ages with lower respiratory tract infections (LRTIs). This study was conducted to detect M. pneumoniae and C. pneumoniae in children with community-acquired LRTIs employing serology, polymerase chain reaction (PCR) and nested PCR analysis. Material and Methods: This study included 75 children with acute LRTIs for detection of M. pneumoniae and C. pneumoniae. Blood was obtained for M. pneumoniae and C. pneumoniae antibodies and nasopharyngeal aspirates for M. pneumoniae PCR and C. pneumoniae nested PCR. Results: M. pneumoniae infection was positive in 9 (64.21%) children aged 2–6 months and in 5 (35.79%) aged 7 months–12 years, and this difference was statistically significant (P = 0.002). C. pneumoniae infection was comparable within the age group and statistically insignificant (P = 0.43). Clinical and radiological profiles of M. pneumoniae- and C. pneumoniae-positive and negative patients were numerically comparable. Serology and PCR together detected M. pneumoniae infection in 14 (18.6%) children. The sensitivity, specificity and positive and negative predictive values of serology were 77.78%, 92.42%, 58.33% and 96.83%, respectively. C. pneumoniae infection was positive in 11 (14.6%) children by serology and nested PCR with 50% sensitivity, 87.67% specificity, 10% positive predictive value and 98.46% negative predictive value. Conclusions: Our study confirms that M. pneumoniae and C. pneumoniae play a significant role in community-acquired LRTIs and a combination of serology and nested PCR is useful for its diagnosis.
中文翻译：
血清学和聚合酶链反应在儿科社区获得性下呼吸道感染中肺炎支原体和肺炎衣原体检测中的诊断效用。
目的：肺炎支原体（ M.pneumoniae ）和肺炎衣原体（ C.pneumoniae ）在所有年龄段的下呼吸道感染（LRTI）儿童中发挥着重要作用。本研究旨在采用血清学、聚合酶链反应 (PCR) 和巢式 PCR 分析来检测患有社区获得性 LRTI 的儿童中的肺炎支原体和肺炎衣原体。材料和方法：本研究纳入了 75 名患有急性 LRTI 的儿童，以检测肺炎支原体和肺炎衣原体。获取血液以检测肺炎支原体和肺炎衣原体抗体，并获取鼻咽抽吸物以检测肺炎支原体PCR 和肺炎衣原体巢式 PCR。结果： 2~6月龄儿童肺炎支原体感染阳性9例（64.21%），7月龄~12岁儿童肺炎支原体感染阳性5例（35.79%），差异有统计学意义（ P =0.002） 。肺炎衣原体感染在年龄组内具有可比性，且统计学上不显着（ P = 0.43）。肺炎支原体和肺炎衣原体阳性和阴性患者的临床和放射学特征在数值上具有可比性。血清学和PCR共同检测出14名（18.6%）儿童肺炎支原体感染。血清学检测的敏感性、特异性、阳性预测值和阴性预测值分别为77.78%、92.42%、58.33%和96.83%。通过血清学和巢式 PCR 检测，11 名儿童（14.6%）肺炎衣原体感染呈阳性，敏感性为 50%，特异性为 87.67%，阳性预测值为 10%，98 例。46% 阴性预测值。结论：我们的研究证实肺炎支原体和肺炎衣原体在社区获得性 LRTI 中发挥重要作用，血清学和巢式 PCR 的结合对其诊断很有用。
更新日期：2020-04-01
Indian Journal of Medical Microbiology ( IF 1.4 ) Pub Date : 2020-04-01 , DOI: 10.4103/ijmm.ijmm_20_145 Sanchit Kumar 1 , Bineeta Kashyap 1 , Surinder Kumar 1 , Seema Kapoor 2
Affiliation
Purpose: Mycoplasma pneumoniae (M. pneumoniae) and Chlamydophila pneumoniae (C. pneumoniae) play a significant role in children of all ages with lower respiratory tract infections (LRTIs). This study was conducted to detect M. pneumoniae and C. pneumoniae in children with community-acquired LRTIs employing serology, polymerase chain reaction (PCR) and nested PCR analysis. Material and Methods: This study included 75 children with acute LRTIs for detection of M. pneumoniae and C. pneumoniae. Blood was obtained for M. pneumoniae and C. pneumoniae antibodies and nasopharyngeal aspirates for M. pneumoniae PCR and C. pneumoniae nested PCR. Results: M. pneumoniae infection was positive in 9 (64.21%) children aged 2–6 months and in 5 (35.79%) aged 7 months–12 years, and this difference was statistically significant (P = 0.002). C. pneumoniae infection was comparable within the age group and statistically insignificant (P = 0.43). Clinical and radiological profiles of M. pneumoniae- and C. pneumoniae-positive and negative patients were numerically comparable. Serology and PCR together detected M. pneumoniae infection in 14 (18.6%) children. The sensitivity, specificity and positive and negative predictive values of serology were 77.78%, 92.42%, 58.33% and 96.83%, respectively. C. pneumoniae infection was positive in 11 (14.6%) children by serology and nested PCR with 50% sensitivity, 87.67% specificity, 10% positive predictive value and 98.46% negative predictive value. Conclusions: Our study confirms that M. pneumoniae and C. pneumoniae play a significant role in community-acquired LRTIs and a combination of serology and nested PCR is useful for its diagnosis.
中文翻译：
血清学和聚合酶链反应在儿科社区获得性下呼吸道感染中肺炎支原体和肺炎衣原体检测中的诊断效用。
目的：肺炎支原体（ M.pneumoniae ）和肺炎衣原体（ C.pneumoniae ）在所有年龄段的下呼吸道感染（LRTI）儿童中发挥着重要作用。本研究旨在采用血清学、聚合酶链反应 (PCR) 和巢式 PCR 分析来检测患有社区获得性 LRTI 的儿童中的肺炎支原体和肺炎衣原体。材料和方法：本研究纳入了 75 名患有急性 LRTI 的儿童，以检测肺炎支原体和肺炎衣原体。获取血液以检测肺炎支原体和肺炎衣原体抗体，并获取鼻咽抽吸物以检测肺炎支原体PCR 和肺炎衣原体巢式 PCR。结果： 2~6月龄儿童肺炎支原体感染阳性9例（64.21%），7月龄~12岁儿童肺炎支原体感染阳性5例（35.79%），差异有统计学意义（ P =0.002） 。肺炎衣原体感染在年龄组内具有可比性，且统计学上不显着（ P = 0.43）。肺炎支原体和肺炎衣原体阳性和阴性患者的临床和放射学特征在数值上具有可比性。血清学和PCR共同检测出14名（18.6%）儿童肺炎支原体感染。血清学检测的敏感性、特异性、阳性预测值和阴性预测值分别为77.78%、92.42%、58.33%和96.83%。通过血清学和巢式 PCR 检测，11 名儿童（14.6%）肺炎衣原体感染呈阳性，敏感性为 50%，特异性为 87.67%，阳性预测值为 10%，98 例。46% 阴性预测值。结论：我们的研究证实肺炎支原体和肺炎衣原体在社区获得性 LRTI 中发挥重要作用，血清学和巢式 PCR 的结合对其诊断很有用。
京公网安备 11010802027423号