Background: Methicillin-resistant Staphylococcus aureus (MRSA) has become more prevalent all over the world and it is important to determine MRSA prevalence and typing in different regions. The present study was carried out to determine the prevalence and frequency of circulating molecular types of MRSA isolates as well as their antibiotics susceptibility in Tabriz and Kerman cities of Iran. Materials and Methods: A total of 230 S. aureus isolates were collected from Tabriz (n=125) and Kerman (n=105) during January to December 2018. MRSA isolates were identified by PCR amplification of nuc and mec A genes. Antibiotic susceptibility of MRSA isolates were determined by Kirby-Bauer disk diffusion method. Multiplex PCR was exploited to detect various types of SCCmec. Results: The MRSA prevalence was 51/125 (40.8%) in Tabriz and 60/105 (57.1%) in Kerman. Overall, 36/51 (70.58%) and 15/51 (29.41%) isolates and 37/60 (61.66%) and 23/60 (38.34%) isolates were isolated from inpatients and outpatients in Tabriz and Kerman, respectively. Almost all of the isolates were resistant to penicillin and all of them were sensitive to linezolid. Thirty five (68.2%) and 34(56.6%) of MRSA isolates in Tabriz and Kerman were determined as MDR, respectively. SCCmec typing showed that the frequent SCCmec type in both Tabriz and Kerman cities was SCCmec III (56.86% and 55%, respectively). Conclusion: The high prevalence of MRSA makes it necessary to revisit the antibiotics administration by physicians. Indeed, periodic evaluation of antibacterial susceptibility patterns of the MRSA strains is required for efficient treatment of MRSA infections.


中文翻译：

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伊朗两个不同地理位置的耐甲氧西林金黄色葡萄球菌临床分离株的基因型和表型特征。



背景：耐甲氧西林金黄色葡萄球菌(MRSA) 在世界范围内变得越来越流行，确定不同地区 MRSA 的流行率和分型非常重要。本研究旨在确定伊朗大不里士市和克尔曼市 MRSA 分离株循环分子类型的流行率和频率及其抗生素敏感性。材料和方法： 2018 年 1 月至 12 月期间，从大不里士 ( n = 125 ) 和克尔曼 ( n = 105 ) 收集了总共 230 株金黄色葡萄球菌分离株。通过 PCR 扩增nuc和mec A 基因来鉴定 MRSA 分离株。 MRSA分离株的抗生素敏感性通过Kirby-Bauer纸片扩散法测定。利用多重 PCR 检测各种类型的 SCC mec 。结果：大不里士的 MRSA 患病率为 51/125 (40.8%)，克尔曼的 MRSA 患病率为 60/105 (57.1%)。总体而言，从大不里士和克尔曼的住院患者和门诊患者中分别分离出 36/51 (70.58%) 和 15/51 (29.41%) 分离株以及 37/60 (61.66%) 和 23/60 (38.34%) 分离株。几乎所有分离株均对青霉素耐药，且全部对利奈唑胺敏感。大不里士和克尔曼分别有 35 株 (68.2%) 和 34 株 (56.6%) MRSA 菌株被确定为 MDR。 SCC mec分型显示，大不里士市和克尔曼市最常见的 SCC mec类型为 SCC mec III（分别为 56.86% 和 55%）。结论： MRSA 的高患病率使得医生有必要重新审视抗生素的使用情况。 事实上，为了有效治疗 MRSA 感染，需要定期评估 MRSA 菌株的抗菌敏感性模式。